课件：《基因芯片技术》基因芯片与医学.ppt

The End Thank you THANK YOU SUCCESS * * 可编辑 Agendia have successfully exploited Agilent microarrays to identify markers of breast cancer prognosis. In comparison with standard histological approaches to making these diagnoses, looking at expression patterns yielded more accurate results. These results are not only useful a possible diagnostic tools but provide a starting point to produce therapies targeted at distinct populations that are classified exclusively by their genomic fingerprint. 78份乳腺癌样品，70个基因进行聚类。 对于上面挑选的70个基因，用另外19份样品进行验证 MicroRNAs (miRNAs) are short, processed, RNA moleculesB22 nucleotides in length that can control gene function through mRNA degradation, translation inhibition or chromatin-based silencing mechanisms (Doench and Sharp, 2004). In humans, about 500 miRNAs have been discovered so far (miRBase, Release 9.1; http://microRNA.sanger.ac.uk/sequences) (Griffiths- Jones et al., 2006). In 2004, Calin et al. (2004) reported that more than half of the then known miRNAs were located in fragile sites on chromosomes. DNA copy number abnormalities in these fragile sites can have a direct impact on the miRNA expression levels (Calin et al., 2004). miRNAs can influence a wide variety of biological processes, including development, proliferation and differentiation (He and Hannon, 2004). Accumulating evidence suggests that miRNAs may act as either tumor suppressors or oncogenes that control growth and apoptosis (Esquela-Kerscher and Slack, 2006). In addition, a number of recent studies have highlighted the potential of miRNA profiles for diagnosis and prognosis of some epithelial tumors (Lu et al., 2005) and hematological malignancies 躁郁症 Bipolar disorder 前面所讲的基因表达谱的检测，exon的检测，以及tiling array用于转录产物的检测，标记样本（target）都是cDNA，而检测SNP，标记的需是基因组DNA 引物要求 不需要修饰和特殊的纯化 Leprosy: 麻风病 Illumina’s genotyping assay is called the GoldenGate Assay It is a high specificity extension and ligation assay that allows the simultaneous analysis of over 1500 loci in a sin