戒毒康酊剂中氢溴酸东莨菪碱及总生物碱的含量测定.PDFVIP

戒毒康酊剂中氢溴酸东莨菪碱及总生物碱的含量测定 徐强，章曙丹，冯瑛(浙江省中药研究所，杭州 310023) 摘要：目的 测定中药戒毒康酊剂中氢溴酸东莨菪碱及总生物碱的含量。方法 采用 Diamonsil C18 柱 (250 mm ×4.6 mm, 5 μm)分离测定氢溴酸东莨菪碱，流动相：以甲醇-0.02 mol·L1 醋酸钠溶液(含 0.02%三乙胺和 0.3%四氢呋喃，用冰醋酸调 1 pH 到 6.2)梯度洗脱；流速 1 mL·min ；检测波长：206 nm 。采用酸性染料比色法测定戒毒康中的总生物碱，0.2% 溴百里 香酚兰溶液显色，420 nm 处测得吸光度。结果 氢溴酸东莨菪碱在 1.0~5.0 μg 内呈良好的线性关系(r=0.999 6) ；平均回 收率为 98.8% ，RSD 为 2.29%(n=6) 。总生物碱在 0.04~0.2 mg 内呈良好的线性关系(r=0.999 8) ；平均回收率为 97.6% ，RSD 为 2.06%(n=6) 。结论 本方法简便、快速、准确、专属性强，可作为戒毒康酊剂的检验方法。 关键词：氢溴酸东莨菪碱；高效液相色谱法；总生物碱 中图分类号：R943.3 文献标志码：B 文章编号：1007-7693(2011)07-0673-04 Determination of Scopolamine Hydrobromide and Total Alkaloid in Jiedukang Tincture XU Qiang, ZHANG Shudan, FENG Ying(Zhejiang Research Institute of Traditional Chinese Medicine, Hangzhou 310023, China) ABSTRACT: OBJECTIVE To establish an HPLC method for determination of contents of scopolamine hydrobromide and total alkaloid in Jiedukang tincture. METHODS A Diamonsil C 18 column(250 mm×4.6 mm, 5 μm) was used to isolate and determine scopolamine hydrobromide. The mobile phase was methanol-0.02 mol·L1 NaAc solution (with 0.02% triethylamine and 0.3% tetrahydrofuran, adjusted to pH 6.2 by HAc). The flow rate was 1 mL·min1. The detection wavelength was set at 206 nm. Total alkaloids in Jiedukang tincture were determined with acid dye colorimetry. 0.2% Bromothymol blue was used as the dye reagent and the wavelength was set at 420 nm. RESULTS The linear range of scopolamine hydrobromide was 1.05.0 μg with a good correlation (r=0.999 6). The average recoveries was 98.8% with RSD of 2.29%(n=6). The total alkaloids showed good linearity within the range of 0.040.2 mg(r=0.999 8), the average recoveries was 97.6% with RSD of 2.06%(n=6). CONCLUSION The method established is se