辅助噬菌体在噬菌体示技术中的应用.pdfVIP

微生物学通报 FEB 20, 2009, 36(2): 261~266 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 专论与综述 辅助噬菌体在噬菌体展示中的 应用及研究进展 杜东霞 张 冉* ( 410081) 摘 要: 噬菌体展示技术是一种将外源肽或蛋白质与特定噬菌体衣壳蛋白相融合，展示于噬菌体 表面来构建蛋白质或多肽文库，并从中筛选目的蛋白、多肽或抗体的基因工程高新技术。噬菌粒/ 辅助噬菌体系统是最常用的噬菌体展示系统，此系统中辅助噬菌体对噬菌粒的复制和组装发挥着 至关重要的作用。本文结合当今该领域的最新研究动态，概述了噬菌粒和辅助噬菌体双基因组系 统，着重介绍了不同辅助噬菌体的特点及其突变机制，并对其应用前景进行了展望，以期为该技 术的进一步完善提供一定的借鉴作用。 关键词: 噬菌体展示, 辅助噬菌体, 噬菌粒, 超感染 Application and Progress of Helper Phage in Phage Display DU Dong-Xia ZHANG Ran* (Department of Microbiology, College of Medicine, Hunan Normal University, Changsha, Hunan 410081, China) Abstract: Phage display is a widely used gene engineering high technology. Through the display of exoge- nous peptides or proteins fused specific coat protein on the surface of phages, it is possible to construct pro- teins or peptides libraries and screen interesting proteins, peptides and antibodies successfully. Most com- monly used phage display technology is phagemid/helper phage system, in which helper phages are essential for the replication and assembly of phagemid particles. In this review, in combination with the newest re- search dynamic status, we summarize phagemid/helper phage double-genome system. We mainly empha- sized the features and mutation mechanisms of different helper phages. We also made some prospects for the future directions, in the meanwhile, we also expect that our experience can provide some help for further maturity of the technology. Keywords: Phage display, Helper phage, Phagemid, Supe