卵叶娃儿藤生物碱的提取及其活性功能研究-营养与食品卫生学专业论文.docxVIP

ABSTRACTABSTRACT ABSTRACT ABSTRACT Tylophora ovata纪indl．)Hookex Steud belongs to Asclepiadaceae．It was a Chinese traditional toxic medicine．111e alkaloid components of Tylophora ovata 口indl．)Hook．ex Steud possessed very strong antitumor activitiesaccording to literature．It has played all important role as an ancient medicine for centuries． Particularly today the medicinal use of Tylophora ovata纪indl．)Hookex Steud was widespread and growing．A wide array of therapeutic effects of Tylophora ovata 仁indl．)Hookex Steud such as antimicrobial，anticancer，have been reposed．It Was well known that Tylophora ovata亿ind．)Hook．ex Steud contains alkaloids such as tylophoridieine A，tylophorinine，O-methyl tylophorinidine and tylophorinidine， etc．All of them were said to have most of the medical effects of Tylophora ovata 纪inat．)Hookex Steud．The main studies and results in the paper were mentioned as the following： 1．The data of nutrition and contents of nutrients in Tylophora ovata纪indl．) Hookex Steud．The results were：(g／100 g，dry mass)water，9．44，flash，6．30，total sugar,2．67；fibre，17．03，protein，14．02，fat，11．32．GC-MS WaS used to study many components in the extracts wim SFE—C02 for the first time．The results showed that Hexadecanoic acid，ethyl ester,n—Hexadecanoic acid，Hexadecanoic acidmethyl ester, 2-Nitro一4·(trifluoromethyl)phenol，8一Methoxy-2一methylquinoline，1-(12一methyl·1一OXO tetradecyl)-1-Pyrrolidine and 1-Phenyl一1-azaspiro[4，5】decane were the main components． 2．The total alkaloids in Tylophora ovata(Lindl．)Hookex Steud．were determined by Bromophenol Blue acid dye colorimetry method．111e suitable conditions for colorimetry were obtained：1．5 mL of Bromophenol Blue acid dye，2 mL of CHCL3 and 4．0 mL of Na2HP03-citric acid at pH 4．2．The color of the treated samples Was stable within 60 min．111e results showed that the developed method with high precision could be simple，correct and reproducible for the determination of the contents oftotal alkaloids in Tylophora ovata陋indl．)Hook．ex Ste