广东淮山多糖的纯化及化学结构鉴定研究-林产化学与工业.PDF

24 Vol. 24 Supplement 2004 8 Chemistry and Industry of Forest Products Aug. 2004 聂凌鸿, 宁正祥 ( 华南理工大学食品与生物工程学院, 广东 广州 510640) NIE L H : ( Dioscoreaf ordii Prain et Burkill) , ( Sevage ) Sephade G- 75 , DFPN-DFPA-DFPN- 1. 261. 002. 87DFPA- ( ) 2. 181. 003. 391. 82 : ; ; : T Q91; Q53; Q949. 718. 27 : A : 0253- 24 17( 2004) S0- 0 10 1- 06 ST UDY ON PURIFICAT ION AND CHEM ICAL ST RUCT URE OF POLYSACCHARIDES FROM DIOSCOREA FORDII NIE Ling-hong, NING Zheng- iang ( College of Food and Biotechnology, South China Univ ersity of Technology, uangzhou 510640, China) Abstract: Polysaccharides of Dioscoreaf ordii Prain et Burkill were e tracted by hot w ater and alkali succeededly. T he polysaccharide in the filtrate were precipitated by ethanol. The protein in the product w ere removed by means of trichloroacetic acid follow ed twice by Sevage method. The polysaccharide was decoloured by active carbon, then purified twice by Sephade G- 75 column chromatography to obtain t he homogeneous polysaccharide DFPN- and DFPA- , and their homogentity w ere e amined wit h paper chromatography, polarimetry and molecular w eight determination. T he units of monosaccharides of DFPN - were identified by paper chromatography and gas chromatography to be mannose, glucose, galactose at ratio 1. 261. 002. 87 after complete hydrolysis by acid. Similarly, the monosaccharides of DFPA- were identified to be mannose, glucose, galactose, galacturonic acid at rat io 2. 181. 003. 391. 82( in combination with determination o