沙棘籽原花青素的提取精制及抗氧化性能研究-制药工程专业论文.docxVIP

沙棘籽原花青素的提取精制及抗氧化性能研究摘要 沙棘籽原花青素的提取精制及抗氧化性能研究 摘要 沙棘是水土保持的优良树种，而且具有很高的药用价值。中国每 年有大量的沙棘果、沙棘籽用于生产沙棘原汁和沙棘油，而含有丰富 的原花青素和黄酮类化合物的果渣、耔渣却被作为饲科或废弃物，极 大地浪费了资源。本课题对沙棘籽中的原花青素进行了提取、精制以 及抗氧化性能的研究。 本文在前人对原花青素研究的基础上，建立了分光光度法测定沙 棘籽原花青素的方法：考察了溶剂脱脂和超临界cO。脱脂对原花青素提 取效果的影响，通过正交试验得出超临界cO：脱脂的最佳工艺条件；在 溶剂筛选、单因素考察的基础上，用正交实验方法确定了沙棘籽原花 青素提取的最佳条件。沙棘籽原花青素精制采用了大孔吸附树脂精制 法，从8种树脂中筛选出AB-8树脂作为精制沙棘籽原花青素的树脂， 实验表明AB一8树脂对沙棘籽原花青素的吸附方式符合Langmuir方 程。属于快速吸附。研究了影响吸附和洗脱效果的因素，如提取液的 预处理方法、上柱液浓度、吸附液流速和洗脱剂浓度等，得到了最佳 吸附和洗脱条件。用精制沙棘籽原花青素进行了小鼠抗氧化实验研究， 结果表明其对实验小鼠三项生化指标测定均为阳性，同葡萄籽原花青 素提取物抗氧化作用相比无显著差异性。 本课题采用的提取和精制工艺简单易行，沙棘籽原花青素产品得 率、纯度和活性都较高，具有广阔的工业前景。 关键词：沙棘籽原花青素，提取，精制，抗氧化 STUDIES STUDIES oN EXTRACTIoN AND PURIFICATIoN oF SEA BUCKTHoRN SEED PRoCYANIDINS AND ANTIoXIDANT PRoPERTY ABSTRACT Sea buckthorn(Hippophae rhamnoides L．ssp．Sinensis Rousi)is a well variety of wild plants to keep water and soil and has a high medicinal value．In China，there are huge amount of sea buckthorn fruits and seeds utilized for producing sea buckthorn beverage and seed oil every year． However，the marc of fruit and seed which contain rich procyanidins and flavonoids are just used as feed or wastes．This is the extremely waste of the natural resources．This paper made the researches on extraction， purification and antioxidant property of procyanidins in sea buckthorn seeds residue． The method of analyzing procyanidins of hippophae by spectrophotometer was founded．The influences of de-fat by solvent and supercritical carbon dioxide fluid extraction on the extractive efficiency was investigated．The optimum conditions of supereritical C02 fluid extraction were determined through the method of orthogonal test．On the base of solvent selection and single factor experiments，the optimum technological conditions were attained by orthogonal experiment． Procyanidins were refined by macroreticular resins．In eight kinds of selected macroreticular resins，AB．8 macroreticular resin was the best． The experiment results showed that the adsorption of the procyanidins wa