辽东栎幼苗的外生菌根合成及其生理效应-植物学专业论文.docxVIP

辽东栎幼苗的外生菌根合成及其生理效应摘 辽东栎幼苗的外生菌根合成及其生理效应 摘 要 1．在温室花盆中播种辽东栎(Quercz∞#aotungens如)种子获得辽尔栎幼苗，并对幼 苗接种外生菌根真菌进行菌根合成试验。所用的外生菌根真菌有：铆钊菇(Gomphidius viscidus)、臭红菇(Russulafoetens)、厚环乳牛肝菌(Suillusgrevillei)、褐环乳牛肝菌(Suillus luteus)、彩色豆马勃(Pisolithus tinctorius)、美味牛肝菌(Boletus edulis)、赭丝膜菌 (Cortinariusr∞W)、土生空团菌(Cenococcumgeophilum)。接种两个月后，在铆钉菇、 臭红菇、厚环乳牛肝菌、褐环乳牛肝菌、彩色豆马勃和美味牛肝菌接种的辽东栎幼苗上 观察到了外生菌根，而赭丝膜菌和土生空团菌接种的辽东栎幼苗上未形成外生菌根。进 一步对形成的外生菌根做组织切片观察，在细根皮层细胞问均形成明显的哈蒂氏网，证 实了6种外生菌根真菌与辽东栎幼苗的共生关系。 2．选择铆钉菇和臭红菇接种，观测了外生菌根对辽东栎幼苗生长的影响。接种外生 菌根对辽东栎幼苗的株高、生物量、光合速率均有显著的促进作用。两种外生菌根混合 接种的效果优于单独接种，且以等量混合接种效果最佳。 关键词：辽东垮白苗，外生菌根合成，生理效应 Ectomycorrhizal Ectomycorrhizal formation and physiological effects on Quercus liaotungensis seedlings Abstract t．Ouemus liaotungensis seedlings were obtained by seeds in pots in greenhouse The ectomycorrhizal formation of Q liaotungensis were studied through ectomycorrhizal fungi including Gomphidius viscidus，Russula foetens，Suillus greviUei,s luteus，Pisofithus tinctorius，Boletus edulis，Cortinarius russlls and Cenococcum geophilum．The ectomycorrhizal roots were observed on the seedlings inoculated by G viscidus，R foetens，s grevillei,S luteus，P tinctorius，B edulis，nor on the seedling inoculated by C rllsslls and C geophilum after inoculating fur two months．In ectomycorrhizal tissue section，clear Hartig net 、ve∞showed in the intercellular space of cortical cells of fine roots，which proved the symbiosis relationship between these 6 species of ectomycorrhizal fungi and seedlings of 9 liaotungensis． 2．The physiological effects of ectomycorrhizal on Q．1iaotungensis seedlings was observed after G viscidus and且foete,Ⅱwere inoculated The ectomycorrhizal apparently helped the promotion ofthe height’biomass and photosynthesis of 9 liaolungensis seedlings． The effects ofthe inoculation by the admixture ofthe two ectomycorrhizal fungi was superior to that of inoculation by any fungus alone，furthermore，the effect of the inoculation of admixture by equal quantity appeared to be optimal． Key words：