声诺维传输eNOS基因抑制高脂喂食兔胸腹主动脉粥样硬化的形成-生物化学与分子生物学专业论文.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文 3 3 Abstract Objective: To investigate the effects of eNOS gene on the progression of thoracoabdominal aorta atherosclerosis in rabbit loaded with high fat diet after eNOS gene delivery by using ultrasound with SonoVue. Methods: We constructed and identified the pEGFP-eNOS recombinant．While the mixture of SonoVue and pEGFP-eNOS was injected though helix vien, the thoracoabdominal aorta of rabbits were sonicated by ultrasound for 3min. The arterial vessels of rabbits were harvested at 2, 7and 14 days after delivery for fluorescence microscopy and Western blot analyses