氟达拉滨、米托蒽醌对多发性骨髓瘤细胞株rpmi8226km新.pdfVIP

2008年第35卷第24期 中国肿瘤临床 ·1419· 氟达拉滨、米托蒽醌对多发性骨髓瘤细胞株 RPMI8226KM3的增殖抑制作用* ① ② 秦 群 肖希斌 谢兆霞 中南大学湘雅医院药剂科 （长沙市 410008） 摘要 目的：观察氟达拉滨、米托蒽醌对两株不同多发性骨髓瘤细胞RPMI8226、KM3体外增殖的抑制作用。 方法：采用MTT法观察1.25～20μg/ml氟达拉滨及联合应用米托蒽醌处理RPMI8226、KM3细胞后其增殖的变化。 结果：氟达拉滨剂量逐渐增大时，对两株多发性骨髓瘤细胞抑制率逐渐增强（P0.01）。氟达拉滨对RPMI8226细胞 的IC 为5.26μg/ml，对KM3细胞的IC 为4.93μg/ml。氟达拉滨联合米托蒽醌对RPMI8226、KM3细胞的增殖抑制作 50 50 用显著强于氟达拉滨或米托蒽醌单独处理组（P0.01）。结论：氟达拉滨对两种不同性质的多发性骨髓瘤细胞株均 有明显的杀伤作用。氟达拉滨联合米托蒽醌能更有效地抑制多发性骨髓瘤细胞的增殖，为临床治疗多发性骨髓瘤 提供实验依据。 关键词 氟达拉滨 米托蒽醌 RPMI8226细胞 KM3细胞 The Effects of Fludarabine and Mitoxantrone on the Proliferation of RPMI8226 and KM3 Cell Lines In Vitro 1 2 3 QIN Qun , XIAO Xibin , XIE Zhaoxia Corresponding author: XIE Zhaoxia, E-mail: xzxchangsha@ 1Department of Pharmacy, Xiangya Hospital of Central South University, Changsha 410008, China 2 Department of Hematology, The Second Affiliated Hospital of Zhejiang University Medical School, Hangzhou 313000, China 3 Department of Hematology, The Second Peoples Hospital of Hunan Province, Changsha 410007, China Objective: To study the in vitro inhibitory effect of fludarabine and mitoxantrone on RPMI8226 and KM3 multiple myeloma cell lines. Methods: MTT assay was used to observe the proliferation of RPMI8226 and KM3 cell lines treated with 1.25-20 μg/ml fludarabine or a combination of fludarabine and mitoxantrone. Results: In the fludarabine group, the proliferation was increasingly inhibited as fludarabine dose was increased