生化工程应用Ecoli大肠杆菌之生长曲线.ppt

Western blotting can detect the specificity protein detect one protein in a mixture of any number of proteins. Method (1) Proteins are separated by gel electrophoresis 檢測流程(續) LCM (Laser Capture Microdissection) * * CHAPTER TWENTY-SIX Figure 26.01 Figure 26.02 SDS polyacrylamide gel electrophoresis (PAGE gel) (2) The proteins are transferred to membrane (nitrocellulose or PVDF) Figure 26.05 Western blotimg (3)blocking (4)add primary antibody (5)add labeled secondary antibody (enzyme .Fluorophore….) two-dimensional polyacrylami