指纹图谱结合一测多评模式在藤茶质量评价中的应用研究.PDFVIP

• 4076 • 中草药 Chinese Traditional and Herbal Drugs 第 47 卷 第 22 期 2016 年 11 月 指纹图谱结合一测多评模式在藤茶质量评价中的应用研究 范 莉，侯小龙，王文清，施春阳，童 庆，方建国* 华中科技大学同济医学院附属同济医院 药学部，湖北 武汉 430030 摘 要：目的 建立不同产地藤茶的 HPLC 指纹图谱与一测多评方法，为藤茶的质量控制提供实验依据。方法 采用 Agilent Eclipse Plus C18 （250 mm ×4.6 mm ，5 μm ）色谱柱，流动相为甲醇-0.5% 甲酸水溶液进行梯度洗脱，检测波长为258 、292 、 369 nm，柱温为 50 ℃，体积流量为 0.8 mL/min，并应用 SPSS 19.0 软件对所得数据进行聚类分析和主成分分析，对不同产 地藤茶样品进行质量分类。结果 建立了不同产地藤茶的 HPLC 指纹图谱，确定了 24 个共有峰，鉴定了 3 个主要共有峰， 分别为二氢杨梅素、杨梅素和杨梅苷，并建立了对不同产地藤茶中二氢杨梅素、杨梅素和杨梅苷的一测多评方法。结论 建 立的结合指纹图谱、模式识别和一测多评法的质量控制模式可应用于藤茶的质量评价。 关键词：藤茶；指纹图谱；模式识别；一测多评；聚类分析；主成分分析 中图分类号：R286.6 文献标志码：A 文章编号：0253 - 2670(2016)22 - 4076 - 06 DOI: 10.7501/j.issn.0253-2670.2016.22.024 Application of fingerprint combined with quantitative analysis of multi-component by single marker in quality evaluation of Ampelopsis grossedentata FAN Li, HOU Xiao-long, WANG Wen-qing, SHI Chun-yang, TONG Qing, FANG Jian-guo Department of Pharmacy, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China Abstract: Objective A new quality evaluation method using HPLC fingerprint coupled with quantitative analysis of multi-components by single marker method was developed for the quality control of Ampelopsis grossedentata . Methods HPLC analysis was performed on an Agilent Eclipse Plus C18 (250 mm × 4.6 mm, 5 μm) column using methanol-0.05% aqueous solution of phosphoric acid as mobile phase at flow rate of 0.8 mL/min. The column temperature was set at 50 ℃ and the detection wavelength was at 258, 292, and 369 nm. The quality analyses of samples from different origins were accomplished by cluster analysis and principal component analysis using SPSS 19.0 statistical software. Results The HPLC fingerprint of A . grossedentata from different regions was established. Twenty-four common peaks were found in the HPLC fingerprint of 14 samples, three important com