皮肤光损伤肿瘤模型的建立-中国实验动物学报.docVIP

皮肤光损伤肿瘤模型的建立 王红丽,陈苑,胡银霞,许华亮,黄仕稳,单孔荣,庄晓玲 [摘要] 目的 模拟人皮肤肿瘤形成的自然环境因素，建立光损伤小鼠皮肤肿瘤模型。方法 采用完全随机的两因素析因设计。选择对光线敏感的BALB/c小鼠，背部皮肤脱毛后UVC照射(56 mJ/cm2)，隔天1次，8周结束；二甲基苯蒽/丙酮液（100μg/200μl）外涂，每周1次，共7周，12周后处死。连续测量并记录小鼠背部肿瘤数量和直径, 描绘时间-荷瘤数动态变化图；皮肤组织病理学观察肿瘤形成的组织细胞形态学改变。结果 单纯UVC照射组无肿瘤出现；UVC+DMBA模型组小鼠6周后背部开始出现肉眼可见的瘤体，第9周荷瘤率达到100%， 11、12周荷瘤数渐趋稳定，肿瘤体积有增大趋势，平均荷瘤数为（4.57±3.0）个，肿瘤平均体积为(44.91±4.6）mm3。而单纯DMBA组荷瘤数第8.5周达高峰，后呈下降趋势，肿瘤自然消退率高，数量不稳定。HE染色、瑞氏染色、基底膜带染色均显示为早期皮肤鳞状细胞癌。结论 DMBA是皮肤肿瘤建模的主要因素，但停止该处理因素后荷瘤数量呈下降趋势，UVC作为一个独立因素，在12周内未能诱导出皮肤肿瘤，但UVC与DMBA联合应用具有交互协同作用，可较快速地建立皮肤肿瘤模型。该模型具有出瘤率高，出瘤整齐，荷瘤量多，除去处理因素后瘤体数量依旧稳定，且体积有增大趋势等特点，为皮肤肿瘤的防治研究提供有价值的实验工具。 [关键词]? 7,12-二甲基苯蒽；短波紫外线；皮肤肿瘤；动物模型 [中图分类号] R 758．2 [文献标识码] A [文章编号] Establishment of skin tumor models induced by photo-damage and 7,12-DMBA in mice WANG Hong-li,CHEN Yuan, HU Yin-xia, XU Hua-liang, HUANG Shi-wen, SHAN Kong-rong, ZHUANG Xiao-ling (Department of dermatology of Clinical Medicine college and first affiliated hospital, Guangdong Pharmaceutical University, Guangzhou 510305, China) [corresponding author] ZENG Kang, E-mail：zengkang2 @ gmail. com [ Abstract] Objective To simulate natural environmental factors of human skin tumor formation to establish photo-damaged skin tumor models in mice. Methods The study was designed by completely randomized factorial design语法错误. BALB / c mice that are light-sensitive were irradiated by UVC(56 mJ/cm2) every other day for 8 weeks and were treated topically with 100 μg of DMBA in 200 μl of acetone once a week until 7 weeks on the dorsal skin area after hair removed. The whole process was 12 weeks. The size and diameter of tumors were observed and measured every week. A dynamic growth-curve of tumor-bearing was depicted. The histopathology features of skin dyed by HE staining, Wright s staining and basement membrane were observed to explore Tumor formation and skin morphologic alteration . Results The mice in model group began to form typical epithelial papilloma at 6 week early and the rate of tumor-bearing wer