生物反应器课件DifferentcellpropertiesSelectcellculturemethod.pptVIP

Agitation Agitation - turbine impeller For unaerated water @ 20 C in a fully baffled tank the following formula applies: P= 4.5 x 10-13 * Di5 * N3 * F Di = Impeller Diameter in Inch N = RPM of Shaft F = One (1) for one impeller (1.8) for two impellers (2.4) for three impellers Agitation - turbine impeller Tip Speed should also be considered because of tendency to shear cells. Do not exceed 1 m/s for cell culture And 5 - 6 m/s for microbial cultures. Bioreactor design considerations Shape ASME Bottoms Hemispherical Bottoms Jacket Design Conventional Di