大孔吸附树脂纯化八角枫根中水杨苷工艺-食品科学.pdfVIP

※工艺技术 食品科学 2012, Vol.33, No.22 99 1,2 1 2 2 段 红 ，翟科峰 ，高贵珍 ，曹稳根 (1.宿州学院特色种植业苗种生产工程技术研究中心，安徽 宿州 234000 ； 2.宿州学院化学与生命科学学院，安徽 宿州 234000) 摘 要：研究大孔树脂纯化八角枫根中水杨苷的最佳工艺条件。以水杨苷的吸附率和解吸附率为评价指标，筛选树 脂种类，并优化吸附和洗脱条件。8种大孔吸附树脂中，HPD-826型大孔树脂对水杨苷具有较好的吸附分离性能， 最佳的纯化工艺条件为 μg/mL 、最大上样量6.5BV、 : 洗脱流速3BV/h，先用4BV 的 上样液质量浓度45.12 径高比1 8、 水洗柱除去水溶性杂质，再用5BV体积分数30% 乙醇溶液洗脱 经HPD-826型大孔树脂处理后的水杨苷回收率可达 。 78%左右 HPD-826大孔树脂对水杨苷纯化的综合性能较好，工艺稳定、可行，适合于工业化生产。 ， 八角枫；水杨苷；HPD-826大孔树脂；纯化 Macroporous Resin Adsorption for Purifi cation of Salicin from Alangium chinense (Lour.) Harms DUAN Hong1,2，ZHAI Ke-feng1，GAO Gui-zhen2 ，CAO Wen-gen2 (1. Engineering Research Center of Special Farm Seed Production, Suzhou University, Suzhou 234000, China； 2. College of Chemistry and Life Science, Scizhou University, Suzhou 234000, China) Abstract ：The present study was conducted to determine optimal conditions for the purifi cation of salicin from the dried roots of Alangium chinense (Lour.) by macroporous resin adsorption. Among 8 types of macroporous resins, HPD-826 was found to be the best one for the adsorption and desorption of salicin. Adsorption and desorption conditions were optimized for the application of HPD-826 to purify salicin from crude extract from the dried roots of Alangium chinense (Lour.). The best purifi cation results were obtained when 6.5 BV of crude extract sample at a concentration 45.12 μg/mL was loaded onto an HPD-826 column with a diameter/height ratio of 1:8 at a fl ow rate of 3 B