石墨烯量子点与钡餐对小鼠肠壁成像的对比研究.doc

PAGE PAGE 4 石墨烯量子点对小鼠小肠成像及微循环血流影响的研究 马永强1，2*，王振国1，苟学立1，李娜1，冯亚强2，柳文娟1，阎兴斌2*，刘 斌1* （兰州大学口腔医学院1，中国科学院兰州化学与物理研究所固体润滑国家重点实验室2，兰州，730000） [摘要] 目的 研究石墨烯量子点（GQDs）对小鼠小肠的成像性能及对小鼠肠系膜微循环的影响。方法 以纯水和CdTe QDs 作为阴性及阳性对照，并与临床上常用的钡餐作对比，采用FX-in vivo成像系统进行GQDs对C57BL/6N小鼠小肠的成像研究；用微循环显微镜观察其对肠系膜微动脉、微静脉和毛细血管的影响。结果 GQDs灌胃1h后紫外光激发下小鼠小肠呈亮绿色充盈影像，肠壁边缘形态及黏膜细微结构清楚，肠系膜血管呈淡绿色影像；钡餐灌胃1h后X线照射下小肠呈高密度团块影像，肠壁黏膜细微结构不清楚，不能显示肠系膜血管。与灌胃前和纯水组相比，GQDs灌胃后小鼠肠系膜微动脉管径增大（P0.05），钡餐灌胃后微动、静脉管径减小（P0.05）；钡餐和QDs灌胃后小鼠肠系膜微动、静脉及毛细血管的血液流速明显加快（P0.05），而GQDs、纯水灌胃后无明显变化。结论 GQDs和钡餐均能对C57BL/6N小鼠小肠成像，GQDs对肠壁及黏膜的成像效果优于钡餐，对小鼠肠系膜微循环的影响较小，在小肠和血管成像方面有良好的应用前景。 [关键词] 石墨烯量子点；钡餐；肠系膜微循环；肠成像 Study on Image Formation of GQDs on Intestinal and Effects on Mesenteric Microcirculation in mice Ma Yongqiang1，Wang Zhengguo1，Gou Xueli1，Li Na1，Feng Yaqiang2，Liu Wenjuan1,Yan Xingbin2，Liu Bin1(1School of Stomatology, Lanzhou University,?2State?Key?Laboratory?of Solid?Lubrication,?Lanzhou?Institute?of?Chemical Physics, Chinese Academy?of Sciences, Lanzhou, Gansu Province, 730000, China) [Abstract] Objective To study image formation of GQDs on intestinal and Effects on mesenteric microcirculation in mice. Methods Image formation of GQDs on intestinal was observed by FX-in vivo imaging system in C57BL/6N mice , compared with X ray barium meal examination. Pure water and CdTe QDs were used as positive and negative control, respectively. The effect of GQDs and barium meal on the diameter of mesenteric arterioles, venules, capillaries and the velocity of blood flow were observed by the mesenteric microcirculation microscope. Results Animals received GQDs by gavage, after 1 h, brightly green images excitated by UV were formed in the mice intestinal .and clear edge of intestinal wall and mucosal subtle structure were revealed. Meanwhile, mesenteric vessels in light green. To barium meal examination, high-density images by X-ray were observed, but intestinal mucosal subtle structure was not clear, mesenteric vessels can not be displayed. For GQDs gr