蔬菜中11种有机磷农药残留的气相色谱检测及其对蔬菜-食品科学.pdf

198 2011, Vol. 32, No. 06 食品科学 ※分析检测 吴 晖，朱 珍，风华亮，李晓凤 * (华南理工大学轻工与食品学院，广东 广州 510640) 建立蔬菜中 11 种农药多残留的气相色谱分析方法。对NY/T 761 — 2008 《蔬菜和水果中有机磷、有机氮、 拟除虫菊酯和氨基甲酸酯类农药多残留的测定》标准的蔬菜前处理方法进行改进，并探讨蔬菜基质对检测的影响。 使用 DB-17 毛细管柱分离，FPD 检测器进行检测。结果显示：11 种有机磷农药分离效果好，在黄瓜、西红柿、 生姜中的 3 个添加水平，回收率为 71.2%～117.9%，相对标准偏差(RSD)为2.1%～11.2%，最低检出限为0.005～ 0.060mg/kg ，保留时间为6.396～19.400min。在各自线性范围内线性关系好(r ＞0.999)；使用40 ℃加热氮吹浓缩， 可分别提高敌敌畏、甲拌磷回收率至 95.00% 和 103.30%；在被测的 13 种蔬菜中，甘蓝、娃娃菜、上海青有少 量杂峰影响农药检出；韭菜有大量的基质峰干扰农药检出。 气相色谱(GC) ；有机磷农药残留；蔬菜；基质 Determination of 11 Organophosphorus Pesticide Residues in Vegetables by Gas Chromatography and Its Effect on Matrix WU Hui ，ZHU Zhen ，FENG Hua-liang ，LI Xiao-feng* (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract：In order to establish a gas chromatography method for 11 kinds of pesticide residues in vegetables, the pre-treatment method for vegetables according to the standard of NY/T 761 －2008 entitled ,,Determination of organic phosphorus, organic nitrogen, pyrethroid and carbamate pesticide residues in fruits and vegetables,,was modified. Meanwhile, the effect of vegetable matrix on determination efficiency of pesticide residues was explored. During the determination of pesticide residues, DB-17 capillary separation column and flame photometric detector (FPD) were used. The results indicated that 11 organophosphorus pesticide residues could be effectively separated. The recovery rates of 11 pesticides in cucumber, tomato and ginger with three spiked levels were in the range of 71.2% －117.9%. The relative standard d