不可逆性电穿孔消融山羊肝脏的组织病理学变化.doc

不可逆性电穿孔消融山羊肝脏的组织病理学变化 刘颖1，熊正爱1，周玮1，李成祥2，姚陈果2，姜蓉3 （1.重庆医科大学附属第二医院妇产科，重庆，400010；2．重庆大学输配电装备及系统安全与新技术国家重点实验室，重庆，400030；3.重庆医科大学干细胞与组织工程研究室，重庆，400016） [摘要]目的 不可逆性电穿孔（irreversible electroporation, IRE）消融山羊肝脏组织后，观察肝细胞形态学及其酶的变化。方法 固定脉宽100μs，频率1Hz，电压2000V，脉冲数120个，彩色超声探头引导电极针经皮定位穿刺处理山羊肝脏组织，于治疗后即刻、24小时、3天、14天处死动物后取材，行苏木素-伊红（HE）染色、葡萄糖-6-磷酸酶（Glucose-6-phosphatse,G-6-P）、琥珀酸脱氢酶（Succinodehydrogenase, SDH）、毛细胆管吡啶银组织化学染色。结果IRE消融后靶区肝细胞完全坏死，与周围正常细胞分界清楚，消融区肝细胞G-6-P与SDH活性明显降低，毛细胆管破坏。结论 IRE可以完全消融靶区肝细胞，使其细胞内的酶功能降低或丧失。其技术可以实现对病变区域具有细胞膜性成分组织非选择性的有效性杀伤，为IRE用于肿瘤的治疗提供了实验依据。 关键词：脉冲电场；不可逆性电穿孔；肝脏；组织病理学 Histopathology research on goat livers with irreversible electroporation Liu Ying1, Xiong Zhengai1, Zhou Wei1, Li Chengxiang2, Yao Chenguo2, Jiang Rong3 (1. Obstetrics and Gynecology department, the 2nd affiliated hospital of Chongqing Medical University, Chongqing, 400010, 2. State Key Laboratory of Power Transmission Equipment System Security and New Technology; Chongqing, 400030, 3. Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing, 400016) 基金项目：国家自然科学基金重点项目Abstract Objective To investigate the histopathology changes of goat livers with percutaneous irreversible electroporation (IRE) treatment. Methods Pulse electric fields (PEFs) with permanent duration (100 μs), frequency (1Hz), voltage (2000V) and pulses (120 pieces) were performed on the electrodes which were placed under ultrasound guidance through the animal skin to the target area. The treated area was observed by hematoxylin and eosin (HE) stain, Glucose-6-phosphatse stain (G-6-P), Succinodehydrogenase(SDH) stain, Cholangioles Pyridine silver stain at the time of 0, 24hours, 3days, 14days after IRE ablation. The function of G-6-P enzyme and SDH, and histological feature of liver cells and cholangioles were all observed by light microscope. Results Complete hepatic cell death was observed after the IRE treatment and there was a sharp demarcation between the ablated zone and the non-abla