第二章 转基因动物发展历程.pptVIP

* Self-inactivating lentiviral vector-derived EGFP transgenic marmosets. a–d, The transgenic marmoset infants are shown. Shown are 584 (Hisui) (a), 587 (Wakaba) (b), 588 (Banko) (c), and twin infants 594 (Kei)/666 (Kou) (d). 584, 587 and 588 contained CAG-EGFP and 594/666 carried CMV-EGFP. Inset boxes in each panel show epifluorescent images of the paw of a transgenic animal (right), compared to a wild-type animal’s foot pad (left). All animals except 588 expressed EGFP in their paw. 666 expressed EGFP at a slightly lower level. 转基因绒猴 皮肤血液会发光.flv * * Figure 1 (A) Engineering zinc-finger nucleases for inducing gene disruption at BLG locus in bovine. (B) Nature of ZFNsinduced mutants in bovine fetus fibroblast cells. About 100 mutants were analyzed, and most of them were short fragment deletions or insertions. Vertical axis indicates the mutant percentage. Horizontal axis indicates the different scale of fragment deletion (?) or insertion (+). (C) To detect mutations in a single-cell colony, the PCR products of a single-cell colony were sequenced directly. If the colony is a mutant clone, the peak after (around) ZFNs cutting site (purple box) should be double peaks (red box). (D) Sequence analysis showed that the cloned cattle is a bi-allelic mutant at the BLG locus. (E) The BLG gene-modified cloned cattle derived from colony 16. * * 1985年TG鱼 黄河鲤 朱作言院士的故事：/tech/2009-09/23/contenthtm * where C is the total energy from food, F is the energy lost in faeces, U is the energy lost in nitrogen excretion, R is the energy channelled to metabolism, and G is the energy channelled to growth. * 小学语文四年级教案——《一定要争气》 童第周克隆童鱼的故事：/n35081/10375975.html 鲫鱼的核植入金鱼卵中 牛满江 美国教授 * 转基因鱼.flv GM Rainbow[VOA].flv 美国：转基因三文鱼上市惹争议.flv * * 初生的小牛各项体征指标正常，叫声洪亮，精神状态很好。遗憾的是，就在动物医学专 家为母牛进行子宫缝合时，小牛发生了意外，饲养人员在给小牛喂食初乳时，不慎将奶呛入 小牛的呼吸道，小牛呼吸困难，虽尽力抢救，还是未能挽住这幼小的生命，它仅存活了1小 时20分钟。 中国农大的动物医学专家齐长明教授对尸体解剖后证实，小牛各器官正常，死亡纯属意外。它的顺利出生，标明我国科学家已完全掌握了世界上只有少数国家掌握的动物体细胞克隆技术。 “九五”期间，课题组就已经成功获得了转基因绵羊6只，这6