毛细管电泳临床分析-分析化学专业论文.docxVIP

中 中 文摘要 采用高效毛细管区带电泳法，首次同时分离分析了糖尿病并发肾 病患者血液和尿液中与肾病相关的肌酸、肌酐、脲和尿酸四种非蛋白 氮代谢产物。在优化的条件下，15 min内可对患者血液和尿液中上述 四种物质同时进行定量。经实际样品验证，方法具有快速、灵敏、重 现性好和需样量小的特点，完全可用于临床上病人血液和尿液的日常 分析和肾病的监测。 采用高效毛细管区带电泳法，研究了糖尿病肾病病人血液红细胞 中的参与氧化应激的氧化型谷胱甘肽(GSSG)和还原型谷胱甘肽 (GSH)的测定方法。对影响分离的条件进行了优化后，可在3 min 内同时对血红细胞中GSSG和GSH进行定量。方法具有较高的灵敏 度，较宽的线性范围和好的重现性，完全可用于临床上患者体内谷胱 甘肽含量的测定、氧化应激水平的监测和病理的研究。 研究了毛细管电泳一乙腈．盐体系在线堆积方法，实现了大体积进 样，提高了检测灵敏度；对影响堆积的参数进行了系统研究和优化， 探讨了堆积的机理，提出乙腈．盐堆积方法是瞬间．准．等速电泳过程； 并在机理的指导下完成了正离子和负离子的同时堆积以及临床上糖 尿病并发肾病患者血液中低含量谷胱甘肽的测定。 关键词：高效毛细管区带电泳，乙腈．盐堆积方法，瞬间．准．等速电泳，糖尿病 肾病，血液样品，尿液样品，血红细胞，非蛋白氮，肌酸，肌酐，脲，尿酸，氧 化应激，谷胱甘肽，『E离子，负离子 AbstractA Abstract A new high-performance capillary zone electrophoresis method assay for creatine，creatinine，urea and uric acid。non—protein nitrogen markers of human diabetic nephropathy(DN)，both in plasma and urine has been developed．Under the optimum condition，the four markers can be separated within 1 5 min．The proposed method has the advantages of good reproducibility,wide linearity and high sensitivity and has been successfully applied to the determination of clinical samples and monitoring DN． A new capillary zone electrophoresis method for separation of reduced form(GSH)and oxidized form(GSSG)glutathione．which were related to oxidative stress．in human red blood cell of DN patient was systematically investigated．GSH and GSSG can be baseline separated within 3min under the optimum conditions．The proposed method provided good reproducibility． high sensitivity and wide linearity and has been successfully applied to the determination of GSH and GSSG in the clinical samples and the status of oxidative stress in patient’S body． Capillary electrophoresis online stacking method——acetonitrile--salts stacking method was studied。a larger volume of sample was introduced to the capillary and a higher sensitivity was successfully obtained．The influences of several experimental factors on the acetonitrile—salts stacking method was also systematically investigated．Based on the experimental r