香雪兰花朵衰老的生理生化分析.pdfVIP

不同品种呼吸高峰出现的时间和变化幅度有所不同；花被片呼吸速率变 化表现出与整朵花一致的规律，但雌蕊的呼吸速率变化因品种不同而 异。相关分析表明，整朵花的代谢强度主要取决于花被片的呼吸强度。 3. 衰老过程中，香雪兰花被片中的可溶性糖和可溶性蛋白质含量在 较早期就开始下降，并且随着衰老的加剧迅速下降至很低的水平。此外， 两个品种之间的蛋白质含量有着显著的差异。相关分析表明，花朵在自 然衰老过程中，细胞内的可溶性糖和可溶性蛋白质的含量变化一致，说 明两者之间没有或者很少发生相互转化。 4. 两个香雪兰品种花被片组织中的 MDA 含量和细胞膜透性均随 着花朵的发育不断上升，而且同一发育级别下，‘上农红台阁’的 MDA 含量要明显高于‘上农金皇后’。细胞膜透性和 MDA 含量之间存在显著 的正相关。 5. 两个品种的香雪兰花被片组织中的 SOD 活性均在发育前期较 高，并随着衰老加剧不断下降，同时，‘上农金皇后’的 SOD 活性要显著 高于‘上农红台阁’。POD 活性在花朵完全盛开前不断增加，花被片萎蔫 后活性迅速下降，并且出现明显下降的时间要晚于 SOD，‘上农金皇后’ 的POD 活性同样要高于‘上农红台阁’。此外，SOD 与衰老相关系数的 绝对值要大于 POD 。 6. ‘上农红台阁’花被片内的花青素含量随着花朵的显色与衰老呈现 出快-慢-快的增长模式。 第 II 页 关键词：香雪兰，花被片，呼吸速率，可溶性糖，可溶性蛋白质，膜脂 过氧化，细胞膜透性，抗氧化酶 第 III 页 STUDIES ON THE PHYSIOLOGICAL AND BIOCHEMISTRY CHANGES OF FREESIAS DURING FLOWER DEVELOPMENTAL PROCESS ABSTRACT Freesia × hybrida, which belongs to Iridaceae family, is one of the famous perennial corm plants, being used as a popular cut flower and an important potted plant around the world. Freesias were valued for their wide color range, flower scent, long vase-life, and beautiful posture. But their tepals were easily wilted and the tepals’ color deteriorated, afftecting their ornamental value. Therefore, how to lengthen the vase life of the Freesias is becoming one of the limits for its further development. The works in this thesis were focused on the respiration changes and several physiological and biochemical factors in two Freesia cultivars, Freesia× hybrida ‘Shangnong Jinhuanghou’ and Freesia× hybrida ‘Shangnong Hongtaige’, during the flower developmental process. The aim of this study is to know the physiological metabolisms of Freesias . The main results showed as follows: 1. The mean vase life of flowers from ‘Shangnong Jinhuanghou’ were