液相荧光法测定水果中壬基酚聚氧乙烯醚及其降解产物-食品科学.PDF

174 2010, Vol. 31, No. 12 食品科学 ※分析检测 吕岱竹 ( 中国热带农业科学院分析测试中心，海南海口 571101) ：为建立超声波提取热带水果中环境激素壬基酚聚氧乙烯醚及降解产物壬基酚、液相荧光法测定残留量的 方法，对超声提取的温度、时间、功率进行条件优化，提取液经净化浓缩后，用C 18 色谱柱进行分离，液相色 谱荧光法进行测定。结果表明：样品中壬基酚聚氧乙烯醚及壬基酚在0.5～50 μg/mL 范围内线性良好，检出限分 别为0.04mg/kg 和0.008mg/kg ，方法回收率分别为92%～102% 和75%～92% 。该方法快速灵敏、准确度高，符合 残留检测的要求。 ：壬基酚聚氧乙烯醚；壬基酚；超声波提取；液相荧光法 Determination of Alkylphenols and Alkylphenol Ethoxylates in Tropical Fruits by LC with Ultrasonic-assisted Extraction . . LU Dai-zhu (Analysis and Testing Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China) Abstract ：A liquid chromatographic (LC) method based on ultrasonic-assisted extraction was presented for the determination of alkylphenols and alkylphenol ethoxylates residues in tropical fruits. The determination of samples by the method developed was achieved through the steps of extraction, condensation, chromatographic separation on a C18 column and fluorescence detection. Optimum conditions for the ultrasonic-assisted extraction of samples were investigated. Calibration curves for alkylphenol ethoxylates and nonyl phenol both exhibited good linearity over the concentration range from 0.5 to 50 μg/mL. Detection limits were 0.04 mg/kg for alkylphenol ethoxylates and 0.008 mg/kg for nonyl phenol. Mean recoveries for alkylphenol ethoxylates and nonyl phenol at 3 spike levels ranged from 92% to 102% and from 75% to 92%, respectively. In conclusion, this method is rapid, sensitive and accurate and meets the requirements of pesticide residue determination. Key words ：alkylphenol ；alkylphenol ethoxylates ；ultrasonic-assisted extrac