自然流产模型小鼠子宫蜕膜组织miRNAs的表达.docVIP

自然流产模型小鼠子宫蜕膜组织miRNAs的表达研究 谭 彬，何俊琳，刘学庆，黎 刚，丁裕斌，陈雪梅，王应雄，余秋波（400016重庆，重庆医科大学生殖生物学研究室） [摘要] 目的 寻找自然流产模型小鼠(CBA/J×DBA/2)子宫蜕膜组织与正常妊娠小鼠(CBA/J×BALB/C)子宫蜕膜组织之间差异表达的miRNAs。方法 建立自然流产小鼠模型和正常妊娠小鼠模型，利用miRCURY LNATM microRNA Arrays 10.0检测两组孕小鼠子宫蜕膜组织miRNAs的表达，筛选两组小鼠子宫蜕膜组织差异表达的miRNAs，并对其进行功能检索和靶基因预测。结果 CBA/J×DBA/2小鼠胚胎吸收率为30.9%，CBA/J×BALB/C小鼠胚胎吸收率为7.0%，两者差异有统计学意义（P0.01）。miRNAs芯片数据表明流产蜕膜组织和正常蜕膜组织之间有13个miRNAs存在明显表达差异（ratios≥2.0或≤0.5），其中表达上调8个，表达下调5个。预测软件显示miR-21和miR-26a的靶基因分别是和妊娠相关的RECK和LIF两个因子。结论 小鼠子宫蜕膜组织miR-21和miR-26a的差异性表达可能在小鼠胚胎自然流产过程中扮演重要角色。 [关键词]自然流产；miRNAs；子宫蜕膜；小鼠 MicroRNAs Expression of microRNAs oin decidua of spontaneous abortion Mmouse modelTan Bin，He Junlin，Liu Xueqing，Li Gang，Ding Yubin，Cheng Xuemei，Wang Yingxiong，Yu Qiubo (Laboratory of Reproductive Biology， Chongqing Medical University， Chongqing 400016 ，China) [Abstract] Objective Differential To observe the differential expression of microRNAs onin decidua betweenof spontaneous abortion mice (CBA/J×DBA/2) and normal pregnant mice (CBA/J×BALB/C). was studied in order to explore the mechanism of spontaneous abortion. Methods Building both A spontaneous abortion mouse model and a normal pregnant miceouse model were established. s，Expression of miRNAs oin decidua fromof the two groups models werewas detected by miRCURY LNA? microRNA Aarrays 10.0. Differential expression of microRNAs was detected by Ffunction analysis and prediction of target geness prediction of the differential expression miRNAs were carried out. Results The embryo resorption rate was significantly higher in the CBA/J×DBA/2 group was significantly higher than in the CBA/J×BALB/C group (30.9% vs 7.0%，P0.01). The data on MmicroRNA chip showed that thirteen differential the expression of 13 miRNAs，including up-regulated and down-regulated expression of 8 and 5 miRNAs (ratios ≥ 2.0 or ≤ 0.5) were was significantly different in detected in decidual tissue betweenof spontaneous abortion mice and normal pregnant mice， including up-regulation of 8 miRNAs