稻纵卷叶螟对43种禾本科植物挥发物的触角电位EAG-应用昆虫学报.doc

·PAGE 480· 应用昆虫学报Chinese Journal of Applied Entomology 53卷 3期 程建军等: 稻纵卷叶螟对43种禾本科植物挥发物的触角电位（EAG）反应 ·PAGE 481· 应用昆虫学报Chinese Journal of Applied Entomology 2016, 53(3): 472?481. DOI: 10.7679/j.issn.2095?1353.2016.062 *资助项目Supported projects：农业公益性行业科研专项（200903051）；江苏省科技项目（现代农业）（BE2015342）；江苏省农业科技自主创新资金项目（CX(15)1057）；扬州市科技计划项目“（2014–22） **第一作者First author，E-mail：391322183@ ***通讯作者Corresponding author，E-mail：liufang@ 收稿日期Received：2016-04-19，接受日期Accepted：2016-06-30 稻纵卷叶螟对43种禾本科植物挥发物的触角电位（EAG）反应* 程建军** 朱 均 刘 芳*** （扬州大学园艺与植物保护学院，扬州 225009） 摘 要 【目的】 为了筛选对稻纵卷叶螟Cnaphalocrocis medinalis成虫有生物活性的植物挥发物，为开发植物引诱剂诱集稻纵卷叶螟提供依据。【方法】 采用触角电位技术（Electroantennogram, EAG）测定了稻纵卷叶螟雌雄蛾对来自禾本科植物的43种挥发物的触角电位反应。【结果】 稻纵卷叶螟雌、雄蛾对50 000 μL/L反-2-己烯醛的EAG反应分别较对照高16.6倍、10.9倍。稻纵卷叶螟雄蛾对50 000 μL/L正戊醛的EAG反应值为（7.347±1.582）mV，是对照的2.6倍；对5 000 μL/L和50 000 μL/L苯甲醛的EAG反应分别较对照高2.0倍、2.4倍；对50 μL/L、500 μL/L水杨酸甲酯的EAG反应较对照分别提高了65.2%、72.1%。【结论】 反-2-己烯醛、正戊醛、苯甲醛、水杨酸甲酯对稻纵卷叶螟具有EAG活性，可能为稻纵卷叶螟植物源引诱剂的主要成分。 关键词 稻纵卷叶螟，禾本科植物挥发物，触角电位反应 EAG response of Cnaphalocrocis medinalis to 43 graminaceous plant volatiles CHENG Jian-Jun** ZHU Jun LIU Fang*** (College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China) Abstract [Objectives] To identify plant volatiles that elicit an electroantennogram (EAG) response in Cnaphalocrocis medinalis in order to develop effective attractants for this pest. [Methods] The olfactory sensitivity of C. medinalis to 43 graminaceous plant volatiles was tested using the EAG technique. [Results] EAG responses of female and male C. medinalis to 50 000 μL/L (E)-2-Hexenal were 16.6 and 10.9 fold higher, respectively, than their responses to the control substance. The magnitude of the EAG response of males to 5 000 μL/L valeraldehyde was (7.347±1.582) mV, which was 2.6 times that of their response to the control. The EAG responses of males to 5 000 μL/L and 50 000 μL/L benzaldehyde were 2.0 and 2.4 times higher, respectively, than thei