多发行硬化治疗方法.ppt

ms-g-s 双向电泳 (2-DE) 等电点聚焦+电泳 银染色法 (系数=1) H3- 放射自显影(系数=4) Harrington MG, Merril CR, Goldman D, Xu X-H, McFarlin DE. Two dimensional electrophoresis of cerebrospinal fluid proteins, in multiple sclerosis and various neurological diseases. Electrophoresis, 1984,5:236-245 双向电泳 (2-DE) 14 例正常志愿者 20 例非炎性神经科病人 17 例炎性神经科病人 22 例MS病人 6 例亚急性硬化性全脑炎 Harrington MG, Merril CR, Goldman D, Xu X-H, McFarlin DE. Two dimensional electrophoresis of cerebrospinal fluid proteins, in multiple sclerosis and various neurological diseases. Electrophoresis, 1984,5:236-245 双向电泳 (2-DE) 用双向电泳于MS病人脑脊液中检测出3个特殊的点 序号 分子量 pH值 1, 16 000 6.2 2, 20 000 5.6-6.6 3, 25 000 6.2-6.6 Harrington MG, Merril CR, Goldman D, Xu X-H, McFarlin DE. Two dimensional electrophoresis of cerebrospinal fluid proteins, in multiple sclerosis and various neurological diseases. Electrophoresis, 1984,5:236-245 对此3点的设想 1.取出此3点 2.分离出此3点的蛋白质 3.用分离出来的蛋白免疫动物 4.纯化出针对此蛋白的抗体 5.用抗体定量测定此蛋白 Harrington MG, Merril CR, Goldman D, Xu X-H, McFarlin DE. Two dimensional electrophoresis of cerebrospinal fluid proteins, in multiple sclerosis and various neurological diseases. Electrophoresis, 1984,5:236-245 脑脊液中免疫球蛋白的定量检测 1.免疫单扩散(Mancini)法 2.火箭免疫电泳 3.酶联免疫吸附试验(Enzyme Linked Immuno-Sorbent Assay, ELISA) 4.放射免疫法(Ra