水产食品中纳米颗粒TiO2与重金属Cd复合污染危害的研究.doc

PAGE 4 食品安全质量检测学报 第8卷 第3期 王芳妹, 等: 水产食品中纳米颗粒TiO2与重金属Cd复合污染危害的研究 PAGE 5 第8卷 第3期 食品安全质量检测学报 Vol. 8 No. 3 2017年3月 Journal of Food Safety and Quality Mar. , 2017 基金项目: 国家质量监督检验检疫总局科技计划项目(2016QK058) Fund: Supported by the State Administration of Quality Supervision, Inspection and Quarantine of Science and Technology Plan Projects (2016QK058) *通讯作者: 钟文涛, 博士, 高级工程师, 主要研究方向为生物安全监测。E-mail: wentao_zhong@ *Corresponding author: ZHONG Wen-Tao, Ph.D, Senior Engineer, Hunan Province Produced Commodity Quality Supervision and Inspection Institute, Changsha 410007, China; Key Laboratory of Protein Chemistry and Development Biology of State Education Ministry of China, College of Life Science, Hunan Normal University, Changsha 410081, China. E-mail: wentao_zhong@ 水产食品中纳米颗粒TiO2与重金属Cd复合污染危害的研究 王芳妹1, 2, 陈谋瑛2, 周雅娜2, 李白玉1, 蒋 伟1, 钟文涛1, 2* (1. 湖南省产商品质量监督检验研究院, 长沙 410007; 2. 湖南师范大学生命科学学院, 蛋白质化学与发育生物学教育部重点实验室, 长沙 410081) 摘 要: 目的 研究纳米颗粒二氧化钛(TiO2NPs)和重金属Cd复合污染对水产食品的生态危害。方法 采用光学立体解剖显微镜观察纳米颗粒TiO2和重金属Cd复合污染处理后斑马鱼的形态变化, 记录染毒48 hpf后的孵化数和染毒96 hpf后的死亡数, 并且利用DCFH-DA探针检测了活性氧含量的变化。结果 与仅TiO2NPs污染相比较, TiO2NPs和重金属Cd复合染毒48 hpf后, 胚胎发生心包水肿, 卵黄囊肿。染毒72 hpf后, 胚胎外包偏少, 并且斑马鱼胚胎的孵化率下降, 死亡率上升, ROS含量升高。结论 本研究可为水产食品业生物安全评价提供参考。 关键词: 纳米颗粒; 二氧化钛; 重金属; Cd; 斑马鱼 Research on pollution hazard of nano particles TiO2 and heavy metal Cd compound in the aquatic food WANG Fang-Mei1, 2, CHEN Mou-Ying2, ZHOU Ya-Na2, LI Bai-Yu1, JIANG Wei1, ZHONG Wen-Tao1, 2* (1. Hunan Province Produced Commodity Quality Supervision and Inspection Institute, Changsha 410007, China;2. Key Laboratory of Protein Chemistry and Development Biology of State Education Ministry of China,College of Life Science, Hunan Normal University, Changsha 410081, China) ABSTRACT: Objective To study the ecological harm of the combined pollution of nanometer particles TiO2 and heavy metals Cd in aquatic products. Methods An optical three-dimensional anatomical microscope was used to observe the morphology changes of zebrafish p