成年斑马鱼OKR行为学分析-遗传.pdf

HEREDITAS (Beijing) 2012 9 , 34(9): 1193―1201 ISSN 0253-9772 技术与方法 DOI: 10.3724/SP.J.1005.2012.01193 成年斑马鱼OKR 行为学分析 1 1 1 1 2 1 黄玉斌 , 邹苏琪 , 殷梧 , 王昆 , 王晗 , 胡兵 1. , 230027; 2. , 215123 作为视功能检测和与视觉有关突变体筛选的方法, 眼动(Optokinetic response, OKR)和视动(Optomoter response, OMR)行为学是简单有效的视功能检测手段, 广泛用于幼年斑马鱼研究中, 而成年斑马鱼OKR 的分析 方法却很少有报道。文章介绍了成年斑马鱼眼动反应诱导方式, 以及使用模板匹配(Pattern match) 的方法程序跟 踪眼部运动, 实现了成年斑马鱼OKR 的定量分析。使用该方法, 检测到斑马鱼双眼视觉区对OKR 行为的产生 具有一定的贡献作用, 并且成年斑马鱼单眼对运动光栅表现出一定的方向敏感性。同样的方法也可适用于幼年 斑马鱼的OKR 行为学分析。利用此方法初步检测到了钟基因period1b 突变体幼鱼的OKR 异常。 成年斑马鱼; OKR; 视动反应; 行为学; 幼鱼; 模板匹配 Analysis of adult zebrafish OKR behavior 1 1 1 1 2 1 HUANG Yu-Bin , ZOU Su-Qi , YIN Wu , WANG Kun , WANG Han , HU Bing 1. CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China; 2. Center for Circadian Clocks, Medical College, Soochow University, Suzhou 215123, China Abstract: As a means of visual function testing and visual related mutants screening, the optokinetic response (OKR) and the optomotor response (OMR) behaviour tests are simple and effective tools for visual functional testing, which have been widely used in studying zebrafish larvae. However, adult zebrafish OKR analysis method is rarely reported. In this study, the methods of inducing adult zebrafish OKR behaviour, as well as tracking the movement of eyes using Pattern Match approaches, are presented. The quantitative measurement of