基于RNAseq技术的宫颈癌与癌旁组织差异表达基因分析.docxVIP

基于RNA-seq技术的宫颈癌与癌旁组织 差异表达基因分析 张志珊蒋燕成陈紫萱陈婉花李爱禄李纯孝 福建医科大学附属泉州第一医院 摘要： 目的探讨宫颈癌的发病机制。方法应用RNA-seq技术分析3对宫颈癌及癌旁组 织转录组，利用生物信息方法对差异基因进行基因本体(GO)分析和通路富集 性分析。结果在宫颈癌组织中发现1755个差界表达基因，其中758个基因表达 上调，997个基因表达下调。功能富集分析表明，差异基因富集于细胞粘附、DNA 损伤有关的信号通路上。且宫颈癌组织中发现RAB22A-BCAS1等融合基因。结论 细胞粘附信号通路、RAB22A-BCAS1融合基因可能与宫颈癌的发生机制有关。 关键词： 宫颈癌;RNA-seq;转录组;差异表达基因;融合基因; 收稿日期：2017-06-22 基金：福建省自然科学基金(编号:2014J01436) Analysis of Differently Expressed Genes of Tumor and Adjacent Non一tumor Tissues from Patients with Cervical Cancer by RNA Sequencing ZHANG Zhishan JIANG Yancheng CHEN Zixuan Quanzhou First Hospital, Fujian Medical University; Abstract： Objective To explore the possible pathogenesy of cervical cancer. Methods RNA sequencing was performed to screen the differently expressed genes of 3 pairs of cervical carcinoma and matched adjacent non-tirnior tissues. The differently expressed genes were identified with gene ontology (GO) analysis and pathway cnrichment emalysis. RcsuIts There were a t otal of 1755 cliff ere ntly expressed genes in the samples, including 758 up-regulated genes and 997 down-regulated genes. These differently expressed genes were enriched in pathway related to cell adhesion and DNA damage. Moreover, RAB22 A-BCAS1 fusion gene was found in cervical ceincer tissues. Conclusion The pathway of cell adhesion and RAB22 A-BCAS1 fusion gene may be related to the tumorigenesis and development of cervical cancer. Keyword： Cervical careinoma; RNA-seq; Transcriptome; Differently expressed gene; Fusion gene; Received： 2017-06-22 宫颈癌是女性最常见的恶性肿瘤之一，人乳头状瘤病毒(HPV)的感染是引起宫 颈癌的重要因素，但并非所有感染HPV的患者都会发展为宫颈癌，只有HPV持续 感染导致细胞的基因组异常才最终导致宫颈癌的发生。本研究拟收集福建医科大 学附属泉州第一医院妇科就诊的宫颈癌患者3例，釆用RNA-Seq技术分析宫颈癌 组织与癌旁组织的转录组，筛选出差异表达基因，进而分析差异表达基因所涉 及的信号通路以及融合基因表达，有利于阐明宫颈癌的发病机制，寻找宫颈癌 特异的分子标志物，同时也为基因药物的开发提供候选的药物作用靶点。 1材料与方法 1.1研究对象 收集福建医科大学附属泉州第一医院妇科就诊的宫颈癌患者3例，术前经未经 放化疗及药物治疗，经病理组织学确诊为宫颈癌IIB期，同时取癌组织和癌旁 组织(距离宫颈癌癌灶边缘＞3 cm),置于RNA保存液中-80°C保存。 1.2 RNA提取及质检 3对癌组织及癌旁组织分别用QIAGEN微量RNA提取试剂盒按操作说明书提取 RNA。琼脂糖凝胶电泳分析RNA的纯度和完整性，Nanodrop检测RNA的纯度(0D 26