2个玉米抗矮花叶病分子标记的有效性评价.docVIP

2个玉米抗矮花叶病分子标记的有效性评价 宋茂兴，瞿 会，闫 伟，张旷野，李凤海，朱 敏，钟雪梅，王宏伟，杜万里，吕香玲 （沈阳农业大学特种玉米研究所，沈阳 110866） 摘要：玉米矮花叶病是玉米重要的病毒病害，培育抗病品种是防治该病最经济有效的方法，常规育种方法与分子育种技术相结合可以大大提高抗病育种的效率。本研究利用前期研究开发的2个分子标记Indel186-9和SCAR112，检测100份常用玉米自交系的标记基因型，结合100份玉米自交系抗性表型鉴定结果进行这2个分子标记辅助选择的有效性分析。结果表明，目前种质资源中高抗病材料较少，亟待进行抗病改良。本试验所用的自交系包括不同血缘，抗源主要来源于PB和四平头种质。Indel186-9标记和SCAR112标记的选择符合率均达到80%，同时使用两者选择符合率达到91.67%，其中抗病选择符合率达到100%。Indel186-9和SCAR112标记分别可以使抗病级别从平均7.26级提高到平均2.4级和平均7.63级提高到平均4.27级。试验证明2个标记均可用于对玉米抗矮花叶病材料的选择，正确组合使用可提高对玉米抗矮花叶病材料的选择效率。 关键词：玉米；矮花叶病；甘蔗花叶病毒（SCMV）；分子标记; 选择效率 Efficiency Evaluation of Two Molecular Markers Linked with Resistance to Maize Dwarf Mosaic Disease SONG Mao-xing，QU Hui，YAN Wei，ZHANG Kuang-ye，LI Feng-hai， ZHU Min，ZHONG Xue-mei, WANG Hong-wei, DU Wan-li, LV Xiang-ling (Special Maize Institute, Shenyang Agricultural University, Shenyang 110866) Abstract：Maize Dwarf Mosaic Disease is a main virus disease of maize, and the most effective way of dealing with the disease is the breeding of resistant varieties. The combination of conventional breeding methods and molecular breeding technology can greatly improve the disease-resistant breeding efficiency. In this study, two molecular markers Indel186-9 and SCAR112 developed in previous study were used to identify the genotype of 100 maize inbred lines, and combining phenotype identification, the validity of two molecular markers was investigated for marker-assisted selection (MAS). The results suggest that there were few highly resistant varieties of the germplasm resources and the resistance is pressingly needed to be improved. Inbred lines used in this experiment were made of various blood, and the resistance materials mainly come from PB and sipingtou germplasm. The selection accuracy of both Indel186-9 and SCAR112 were up to 80%, and the selection accuracy of the two markers used simultaneously for MAS was up to 91.67%，among which the resistant selection accuracy was up to 100%. The two markers improved the average resistant level from