Frozen section technique 2英文学习资料 .pdfVIP

Frozen section technique I I Home | Apparatus | Face down cryoembedding | Frozen block embedding | Bone Vise | Price List | Company Story | Frozen section technique | Surg path offerings | references | Gallery of possibilities | Links | Contact Back | Next Frozen section technique | FS technique I I | FS technique III | FS technique IV Click on thumbnail for larger picture Orienting the tissue to the knife This is an extremely important aspect of the frozen section preparation. Using the embedding system described in the previous articles it is quite easy to prepare and orient tissues in the wells so that the knife meets the tissue in a specific orientation. Based on my experience I have come up with certain rules I try to follow: 1) Fat should be the last thing to hit the blade or should hit the blade by itself whenever possible. Fat does not get hard enough to cut well at temperatures that are best for cutting most other tissues. When fat hits the blade before the more manageable tissues it may smear and ruin the rest of the section. I find by hitting the knife last or by itself fat wont interfere with the other tissues as much. The worst situation in my experience is to have fat meet the blade first without a handle of embedding medium. The handle gives the section a start. Without this handle the fat will smear and wipe out the whole section. Sectioning fat will be discussed in greater detail below. If I find myself having difficulty getting a good section because fat appears in the plane I suggest rotating the chuck to avoid the fat or try The Gouge. 2) The most critical aspect of the tissue should be perpendicular or diagonal to the blade and not the first or last aspect of the tissue to hit the blade. Lets Frozen section technique I I consi