涩柿树皮单宁的纯化及抗氧化活性.pdf

50 2016, Vol.37, No.07 食品科学 ※基础研究 涩柿树皮单宁的纯化及抗氧化活性 杨琼琼，李 东，屈 恋，张 强，刘雄民* （广西大学化学化工学院，广西南宁 530004 ） 摘  要：选用超声波法从涩柿树皮中提取单宁，溶剂法、大孔树脂法纯化粗提物，并考察了不同纯度的单宁对 1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH ）自由基和羟自由基（·OH ）的清除作用。结果表 明：涩柿树皮单宁的超声波提取率为5.6%；大孔吸附树脂纯化涩柿树皮单宁的最佳工艺参数为：以HPD-500树脂为 吸附树脂，上柱流速1.5 mL/min，上样质量浓度1.2 mg/mL；洗脱流速1.5 mL/min，乙醇体积分数60%；粗提物、乙 酸乙酯萃取物、大孔树脂纯化物、乙酸乙酯萃余物经大孔树脂纯化后产物、乙酸乙酯萃取物经大孔树脂纯化后产物 的纯度分别为5.6%、11.5%、19.4%、47.4%、53.3%；当质量浓度为1 mg/mL时各纯度单宁对DPPH 自由基有最大清 除率，清除率分别为69.07%、80.19%、92.96%、94.02%、94.05%；清除DPPH 自由基的IC50值分别为0.68 、0.52 、 0.13 、0.12 、0.11 mg/mL ；当质量浓度为0.9 mg/mL 时各纯度单宁对·OH有最大清除率，清除率分别为43.04%、 73.99%、83.00%、94.68%、96.23%。 关键词：单宁；纯化；抗氧化活性 Purification and Antioxidant Activity of Tannin from Persimmon Tree Bark * YANG Qiongqiong, LI Dong, QU Lian, ZHANG Qiang, LIU Xiongmin (School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China) Abstract: Tannin in persimmon tree bark was ultrasonically extracted. The crude extract was purified by organic solvent extraction and macroporous resin adsorption. The free radical scavenging activity of tannin samples with different purities was studied using two different systems. The extraction yield of tannin in the crude extract was 5.6%. The optimum conditions for purifying the crude extract with HPD-500 macroporous resin were as follows: a sample containing 1.2 mg/mL tannin was loaded onto the column at a flow rate of 1.5 mL/min and eluted with 60% ethanol at a flow rate of 1.5 mL/min. The purity of the crude extract was 5.6%, which was increased to 19.4% after macroporous resin purific