毕业论文--人类白细胞抗原HLA-B27与强直性脊柱炎的相关性及两种检测方法的比较.docVIP

人类白细胞抗原HLA-B27与强直性脊柱炎的相关性及两种检测方法的比较 PAGE 6 PAGE 7 人类白细胞抗原HLA-B27与强直性脊柱炎的相关性及两种检测方法的比较 摘要：目的 探讨人类白细胞抗原B27（HLA-B27）的阳性率与强直性脊柱炎（AS）的相关性，并对两种检测方法进行比较。方法 首先用酶联免疫吸附法（ELISA）分别对78名AS患者、172名疑似AS患者及158名非AS患者分别进行检测，计算HLA-B27的阳性率；然后对172名疑似AS患者再用微量淋巴细胞毒试验（MLCT）法进行HLA-B27检测，比较两种方法的检测结果，从而找出更合理的方法。结果 经检测，78名AS患者、172名疑似AS患者及158名非AS患者的HLA-B27的阳性率分别为96.15%、34.88%、5.70%，三组结果有显著性差异(卡方检验，P0.05)；172名疑似AS患者中，ELISA与MLCT均阳性者56人，均阴性者91人，前阳后阴者15人，前阴后阳者10人，两种方法检测结果无统计学意义(卡方检验，x2=0.64，P0.05)。结论 HLA-B27阳性与AS密切相关，是诊断AS的重要依据；ELISA法较MLCT法对HLA-B27的阳性检出率稍高，但无明显差异，而MLCT法无需特殊仪器，且费用较低，故适用于基层医疗结构。 关键词：HLA-B27；强直性脊柱炎；酶联免疫吸附法；微量淋巴细胞毒试验（MLCT） Correlation between HLA-B27 and Ankylosing Spondylitis and comparation of two detective methods Abstract: Objective To investigate the relationship between HLA-B27 and ankylosing spondylitis (AS) and compare the two kinds of detective methods. Methods ELISA was used to detect HLA-B27 antigen in 78 cases of AS patients, 172 cases of suspected AS patients and 158 non AS patients, calculate the positive rate of HLA-B27; then Microlymphocytotoxicity Test (MLCT) was also used to detect the 172 cases of suspected AS patients and compare the detective results and find a more reasonnable method. Result The positive rate of HLA-B27 in 78 patients diagnosed with AS is 96.15%, in 172 patients with suspected AS is 34.88% and in 158 non AS patients is 5.70%, which has significant difference (P0.05); In 172 cases of suspected AS patients, 56 is both positive detected by ELISA and MLCT, 91 is both negative, 15 is positive detected by ELISA and negative by MLCT, 10 is negative by ELISA and positive by MLCT, there are no statistically significances between ELISA and MLCT (Chi-square test, x2=0.64, P0.05). Conclusion HLA-B27 positive rate is closely related with AS, and is important evidence to diagnose AS. ELISA is more sensitive than MLCT in HLA-B27 positive detecting, but without significant difference (P0.05); As MLCT does not need special instrument and the cost is relatively l