大肠癌PARG与PARP关系的初步研究.doc

大肠癌中PARG对PARP的调节作用及意义 李 佳，林 玲，王娅兰（重庆医科大学基础医学院病理学教研室，重庆400016） 摘 要：目的 初步探讨大肠癌中聚(腺苷二磷酸核糖)水解酶[Poly(ADP-ribose) glycohydrolase, PARG]与聚(腺苷二磷酸核糖)聚合酶[Poly(ADP-ribose) polymerase, PARP]的关系。方法 应用S-P免疫组化检测人大肠癌组织中PARG、PARP的表达；以PARG抑制剂丹宁酸（Gallotannin，GLTN）为处理因素，采用流式细胞计数检测小鼠结肠腺癌CT26细胞中PARG、PARP表达变化。结果 PARG和PARP在人大肠癌组织中的表达呈正相关（r =0.30001，P0.05）；流式细胞计数显示，用GLTN处理后，小鼠结肠腺癌CT26细胞中PARG、PARP的阳性细胞率分别较对照组降低，差异具有统计学意义（P0.05）。结论 大肠癌中PARG和PARP的表达有相关性，PARG抑制剂可以抑制PARG及PARP表达，PARG可能对PARP具有调节作用。 关键词：聚(腺苷二磷酸核糖)水解酶；聚(腺苷二磷酸核糖)聚合酶；大肠癌 中图法分类号：R73 文献标识码：A Regulatingory Effects effects of PARG on PARP in Colorectal colorectal Carcinomacarcinoma LI Jia, LIN Ling, WANG Ya-lan (Department of Pathology, Chongqing Medical University, Chongqing 400016, China)Department of Pathology, Chongqing Medical University , Chongqing 400016,China） Abstract: Objective To study the relationship between Poly(ADP-ribose) glycohydrolase (PARG) PARG and Poly(ADP-ribose) polymerase (PARP) PARP in colorectal carcinoma. Methods Immunohistochemical S-P staining was used to examine the expressions of PARG, and PARP in human colorectal carcinoma specimens. The The positive rate of PARG and PARP in murine colon carcinoma CT26 cells was detected by Flow flow Cytometry cytometry(FCM) before and after the treatment of PARG inhibitor Gallotannin. Results The correlation of PARG and PARP expressed in colorectal carcinoma specimens was of positive correlation (r=0.30001, P0.05). The expressions of PARG and PARP were weaker in GallotanninGLTN-treated CT26 cells groups than those in control group cells (P0.05). Conclusion The data suggests that tThe expressions of PARG and PARP are correlated positively in colorectal carcinoma. PARG inhibitor may inhibits the expressions of both PARG and PARP. , indicating that PARG may play a role in regulating regulate the expression of PARP. Key words: Poly(ADP-ribose) glycohydrolase; Poly(ADP-ribose) polymerase; colorectal carcinoma 在细胞内众多蛋白质的聚ADP糖基化过程中，聚(腺苷二磷酸核糖)聚合酶[Poly(ADP-ribo