葡聚糖检测方法.docxVIP

葡聚糖检测方法（试剂盒方法翻译） 提供试剂 瓶1：exo-1,3-β-Glucanase (100 U/mL) plus β-Glucosidase(20 U/mL) suspension, 2.0 mL 瓶2：Amyloglucosidase (1630 U/mL) plus invertase(500 U/mL) solution in 50 % v/v glycerol, 20 mL 瓶3：GOPOD Reagent Buffer. Buffer (48 mL,pH 7.4), p-hydroxybenzoic acid and sodium azide(0.4 % w/v). 瓶4：GOPOD Reagent Enzymes. Glucose oxidaseplus peroxidase and 4-aminoantipyrine. Freeze-dried powder. 瓶5：D-Glucose standard solution (5 mL, 1.00 mg/mL) in0.2 % w/v benzoic acid 瓶6：Control yeast β-glucan preparation ( 2 g, β-glucan content stated on the bottle label). 提供试剂的处理 向瓶1中加入8ml醋酸钠缓冲液，分装-20℃存放。 直接