Lab培养基配制和灭菌.pptVIP

实验一 培养基的配制与灭菌 一 教学要求 通过配制牛肉膏蛋白胨等培养基，掌握配制培养基的一般方法和步骤。 了解采用的灭菌方法和原理，掌握高压蒸汽灭菌技术。 二 实验原理 1、Media Types Solid Media,semi-solid Media, liquid Media . Enriched Media, Selective Media, Differential Media， combination selective and differential media 2 Sterilization – destruction of all forms of microbial life, including endospores.  Physical Methods —Moist heat Autoclaving ??? Action - protein denaturation. ??? Very effective method of sterilization; at about 15 psi（Ib/in2 ） of pressure (121oC [ 250oF]), all vegetative cells and their endospores are killed in about 15 min. ? —Dry heat Di