标准、规范、准则_MFLP-72 The Isolation and Identification of Vibrio cholerae 01 and non-01 from Foods.pdf

Government of Canada Gouvernement du Canada Laboratory Procedure MFLP-72 April 1995 HEALTH PROTECTION BRANCH OTTAWA THE ISOLATION AND IDENTIFICATION OF Vibrio cholerae O1 AND NON-O1 FROM FOODS S. Stavric and B. Buchanan Research Division, Bureau of Microbial Hazards, Food Directorate, Health Canada, Ottawa ON, K1A 0L2 1. APPLICATION This method is applicable to the detection of Vibrio cholerae O1 and non-O1 in foods to determine compliance with the requirements of Sections 4 and 7 of the Food and Drugs Act. 2. DESCRIPTION This method has been shown to produce satisfactory results with fish, seafood, vegetables and coconut milk. V. cholerae classification is based upon agglutination in antisera directed against the somatic O antigens. Species that agglutinate in anti-O1 serum form serogroup O1, while those that do not, form serogroup non-O1 (9.3). Both O1 and non-O1 serogroups include strains that produce cholera toxin. Serogroup O1 is further divided into two biotypes, Classical and El Tor, based upon certain physiological and bacteriophage sensitivity patterns; and three serotypes, Inaba, Ogawa, or Hikojima, based on agglutination with specific antisera (9.5, 9.7). Strains isolated from the 1992/93 epidemic in India and Bangladesh were assigned a new serogroup O139, with a synonym Bengal (9.8). They can be identified by performing a battery of biochemical