流式细胞术在血液病中的应用进展.pdfVIP

流式细胞术在血液病中的应用进展 （selected topics） 艾迪康医学检验中心 夏成青 主要内容 骨髓增生异常综合征 急性白血病微小残留检测 混合表型白血病的诊断 TCR Vβ在T细胞肿瘤诊断中的应用 流式细胞免疫分型的局限性 PNH 骨髓增生异常综合征 急性白血病微小残留检测 混合表型白血病的诊断 TCR Vβ在T细胞肿瘤诊断中的应用 流式细胞免疫分型的局限性 PNH 骨髓增生异常综合征 免 疫 表 型 常 见 异 常 情 况 骨髓增生异常综合征—粒系发育异常 骨髓增生异常综合征--粒细胞发育异常 (CD11b−/CD16−→CD11b+/CD16[+]→CD11b+/CD16+→CD11b++/CD16++) was observed. Deviation from normal was defined as a difference that amounted to at least a half-log signal intensity in 1 parameter, although it must be recognized that the assessment is always at least partially subjective. 骨髓增生异常综合征--粒细胞发育异常 Granulocytes were evaluated for SSC signal, abnormal CD13/CD16,CD56,CD33,CD64. The CD13/CD16 expression pattern was considered abnormal if a deviation of normal granulocytic maturation (CD13+/CD16−→CD13[+]/CD16−→CD13−/CD16[+]→CD13−/CD16+→CD13+/CD16++) was observed (Fig. 1). Also, the CD11b/CD16 expression pattern was considered abnormal if a deviation of normal granulocytic maturation (CD11b−/CD16−→CD11b+/CD16[+]→CD11b+/CD16+→CD11b++/CD16++) was observed. Cancer Volume 116, Issue 19, pages 4549–4563, 1 October 2010 骨髓增生异常综合征—单核细胞发育异常 (Left) Monocytes in normal bone marrow (BM) revealed no expression of cluster of differentiation 56 (CD56) (neural cell adhesion molecule). (Right) In myelodysplastic syndrome (MDS), strong CD56 expression was observed Cancer Volume 116, Issue 19, pages 4549–4563, 1 October 2010 . 骨髓增生异常综合征—红系发育异常 骨髓增生异常综合征—红系细胞发育异常 • Figure 3. (Left) In normal bone marrow (BM), the majority of erythrocytes had strong expression of cluster of differentiation 71 (CD71) (transferrin receptor 1) with some degree of heterogeneity for dim expression. (Right) In myelodysplastic syndrome (MDS), negative CD71 expr