高效液相色谱-电喷雾串联四极杆质谱定性分析双黄连粉针中化学成分.pdf

药学学报 Acta Pharmaceutica Sinica 2009, 44 ( 12) : 1391 1396 ·1391 · 高效液相色谱-电喷雾串联四极杆质谱定性分析双黄连粉针中 化学成分及其药味归属 1* 2 2 罗奇志 , 罗佳波 , 王有志 (南方医科大学 1. 分析测试中心, 2. 中医 学院, 广东 广州5 10515) 摘要: 采用液相色谱-电喷雾串联四极杆质谱法分析双黄连粉针中的化学成分, 并对各化学成分的 味进行 归属。采用C 18 柱, 以甲醇-0.25%醋酸水溶液梯度洗脱, 紫外光谱和电喷雾质谱同时在线检测, 负离子扫描模式 扫描, 对每一组分峰进行质谱分析。通过质谱分析及文献对照, 从双黄连粉针图谱中共准确推 43 个峰的相对 分子质量, 推导出20 个化合物, 并对各化合物进行了 味归属。 关键词: 双黄连粉针; 绿原酸; 黄芩苷; 连翘酯苷; 高效液相色谱-电喷雾串联质谱 中图分类号: R917 文献标识码:A 文章编号: 05 13-4870 (2009) 12-1391-06 Qualitative analysis of the main chemical constituents of Shuanghuanglian injection powder and their origin by HPLC-ESI/MS/MS spectrometry 1* 2 2 LUO Qi-zhi , LUO Jia-bo , WANG You-zhi (1. Analy tical Center, 2. College of Traditional Chinese M edicine, Southern Medical University, Guangzhou 510515, China) Abstract: This paper is to report the analysis of the main chemical constituents of Shuanghuanglian inj ection powder and determination of their origin . The sample solution was analyzed by a Zorbax C 18 column with a gradient mobile phase comprised of methanol and 0 .25% acetic acid solution . Both UV and electrospray ionization mass spectrometry detector were used simultaneously, -Q 1-scan detection mode was evaluated for the identification of the LC peaks. To analyze the mass spectrum of every LC peaks, 43 molecular mass from the ion chromatogram of Shuanghuanglian inj ection powder were identified and among them, structure of 20 compounds were el