限制性核酸内切酶(双语).ppt

Restriction enzymes (限制性核酸内切酶）;Bacterial enzymes which cut DNA into defined and reproducible fragments Identified in the late 1960s Key discovery which allowed the DNA cloning to become a reality ;;限制酶—Cut;Restriction endonuclease(origination);Types of Restriction endonuclease;Type II Recognition sequence characteristics （识别序列特点）—— palindrome（回文结构） ;Bam HⅠ;5’ protruding ends;Enzyme and connection （酶切与连接）;Common enzyme ;nomenclature（命名）;同尾酶 ; isochizomer (同功异源酶) ;Thank you