糖检测准确性的因素分析及病案讨论.pptVIP

额数 GDH-FAD的化学反应 拜耳的试纸是一个应用以下方法的电化学系统：1）当血液进入检测室并溶解酶（葡萄糖脱氢酶）和反应中间体（铁氰化物）时，酶催化的葡萄糖传递电子给铁氰化物的反应就会开始，生成还原性的亚铁氰化物分子；2）血糖仪为工作电极和反电极之间加电压，导致铁氰化物又被氧化以及电子在电极之间流动。由此产生的电流被血糖仪检测并折算成葡萄糖浓度。 GDH-FAD chemistry Bayer’s test strip is an electrochemical system that employs the following method: 1) when blood enters the test chamber and dissolves the enzyme (glucose dehydrogenase) and the mediator (ferricyanide) a chemical reaction occurs in which the enzyme facilitates the passing of electrons from glucose to ferricyanide, creating the reduced molecule ferrocyanide; 2) the meter applies an electric potential between the working and counter electrodes, causing ferrocyanide to be oxidized and electrons to flow through the electrodes. The resulting current is measured and interpreted by the meter algorithm as glucose concentration. GDH-FAD的化学反应 FAD作为辅酶与蛋白质结合在一起，并且负责将电子从葡萄糖传递给反应中间体-铁氰化物。不同于PQQ，FAD不会将电子传递给麦芽糖或半乳糖。也不会受到样本中氧浓度的影响。 GDH-FAD chemistry The FAD cofactor is bound to the protein and is responsible for the transport of electrons from glucose to the mediator, ferricyanide. Unlike PQQ (pyrrolo-quinoline quinone), FAD will not transport electrons from maltose or galactose. It is also unaffected by oxygen. * * Meter to meter; samples spiked with and without maltose Typical values; for maltose peritoneal dialysis around 120 mg/dL (~80% bias) Galactose ; neonates with galactosemia can be at 120 mg/dL * 试纸的化学成分：一个关键因素 在电化学血糖检测系统中，试纸中的酶起着催化葡萄糖和反应中间体分子之间反应的作用。在检测末期，血糖仪向工作电极加上电压。驱动反应中间体中的电子到电极上形成电流并被检测到。 Strip chemistry: a key factor In electrochemical blood glucose monitoring systems, an enzyme in the test strip facilitates the chemical reaction between glucose and a mediator molecule. At the end of the test time, the meter applies an electrical potential to the working electrode. The electrons built up in the mediator molecule are drawn into the electrode and an electrical current is measured. * 其他影响准确度的物质 这张表展示了其他可能的干扰物和来源，以及最高浓度限制。对于扑热息痛（对乙酰氨基酚），临床治疗浓度为1-3mg/dL，高于20mg/