第三讲-酶学基础.pptVIP

作图法处理数据 1、Lineweaver-Burk法 注意底物浓度范围，一般应在Km附近 2、Hanes-Woolf作图：等距离S Woolf-Augustinsson-Hofstee作图 Eadie-Scatchard作图 米氏方程的积分形式及其对数据的处理 上述作图限于初速度阶段：底物转化率小于5％ 产物浓度过低难以测定；或产物根本不能直接测定 重排反应方程式： 积分得： 一个S下进行测定即可作图，求取动力学参数 * Lyases which involve elimination reactions in which a group of atoms is removed from the substrate. This includes the aldolases, decarboxylases, dehydratases and some pectinases but does not include hydrolases. For example: histidine ammonia-lyase (EC 4.3.1.3, systematic name, L-histidine ammonia-lyase; also called histidase). [1.4] ? L-histid