高通量筛选之痛——泛活性筛选干扰化合物Painofhigh-throughput.pdf

药学学报 Acta Pharmaceutica Sinica 20 15, 50 (8): 925 930 ·925 · 通量筛选之痛 泛活性筛选干扰化合物 涛, 杜冠华* ( 中国医学科学院药物研究所, 北京市 物靶点研究与新 筛选重点实验室, 北京 100050) 摘要: 高通量筛选技术在新 发现中已经成为常规的方法, 该技术可以有效地筛选出作用于特定靶点的化 合物, 同时, 这一方法也引入了一些干扰筛选的特殊化合物分子。泛活性筛选干扰化合物以干扰生化实验检测、 对蛋白进行共价修饰、自身形成聚集体等方式干扰 物筛选, 清除此类化合物是重要的研究内容。本文对泛 活性筛选干扰化合物的概念和分类进行了介绍, 并对其产生原因以及相关对策进行综述, 以期提高高通量药物 筛选的技术水平和命中率。 关键词: 泛活性筛选干扰化合物; 高通量筛选; 药物发现; 命中率 中图分类号: R965 文献标识码: A 文章编号: 0513-4870 (20 15) 08-0925-06 Pain of high-throughput screening pan assay interference compounds * XIE Tao, DU Guan-hua (Beijing Key Laboratory of D rug Targets Identification and Drug Screening, Institute of Materia Medica, Chinese Ac ademy of Medical Sciences, Beijing 100050, China ) Abstract : High-throughput screening is a regular approach available for identifying new lead compounds for the growing validated drug targets in drug screening. However, it has also introduced a large number of peculiar molecules which interfere drug screening. Pan assay interference compounds (PAINS) interfere with the progress of drug screening in various ways, such as interfering with a biochemical assay, modifying the protein, aggregate-based inhibitors and so on. So it is of vital significance to remove them. This paper has consulted the concept, category of PAINS and reviewed the way of PAINS interfering and the countermeasures to cope with them to direct the approach of high through sc