高良姜素的含量-食品安全质量检测学报.pdf

3 4 Vol. 3 No. 4 2012 8 Journal of Food Safety and Quality Aug. , 2012 * 孙艳芳, 肖白曼, 陈彦凤, 付 宇, 丁笑莉 (, 150030) 摘 要: 目的 方法 C18 , 268 nm 结果 95.5%~100.8%, RSD 1.1%~2.0%, 95.2%~100.5%, RSD 1.6%~2.8%结论 , , 关键词: ; ; ; Determination of chrysin and galangin in propolis health care foods by reversed-phase high pressure liquid chromatography * SUN Yan-Fang, XIAO Bai-Man , CHEN Yan-Feng, FU Yu, DING Xiao-Li (Heilongjiang Province Center for Disease Control and Prevention, Harbin 150030, China) ABSTRACT: Objective To establish a method for the detection of chrysin and galangin in propolis health care foods by reversed-phase high pressure liquid chromatography (RP-HPLC). Methods Chrysin and galangin in samples were reflux extracted with methanol in water bath, separated by C18 reversed phase column, and detected at wavelength of 268 nm. Results The recovery of chrysin was 95.6%~100.8% with the relative standard deviations of 1.1%~2.0%, and the recovery of galangin was 95.2%~100.5% with the relative standard deviations of 1.6%~2.8%. Conclusion The method is simple, rapid, accurate and reliable, which could be used for the detection of chrysin and galangin in propolis health care foods. KEY WORDS: chrysin; galangin; reversed-phase high pressure liquid chromatography; health care foods [2] 1 引 言 [3] Apis mellifera L. [4] ,