基因打靶TALEN技术-百替生物.pptVIP

基因打靶 ——TALEN 正向遗传学 VS 反向遗传学 目前常用的遗传学技术 Forward genetics:Chemical mutagenesis (EMS,ENU)Insertional mutagenesis(retrovirus, transposon)Gene trap(transposon)Natural mutation screen Phenocopy (transient effect):RNAiMorpholino(MO) antisense oligonucleotides Reverse genetics:Knock out by HR: via plasmid in ES cell (mouse) via rAAV Gene targeting by ZFN and TALEN The short story of gene silencing — the main characteristics of short RNAs Short interfering RNAs — post-transcriptional gene silencing Morpholino structure and functions 细胞内的稳定性 基因表达敲低的效率 细胞毒性 免疫应答 进入细胞的方式 是否可遗传 RN