運用微晶片篩選癌症基因和探討其上游之調控讲义.pptVIP

運用微晶片篩選癌症基因和探討其上游之調控;Contents;Central dogma of molecular biology;Types of RNAs;癌症的形成及97年台灣前十大主要癌症死亡原因摘要;By Hanne Jarmer, BioCentrum-DTU, Technical University of Denmark;Microarrays are used to measure gene expression levels in two different conditions. Green label for the control sample and a red one for the experimental sample. DNA-cDNA or DNA-mRNA hybridization.The hybridised microarray is excited by a laser and scanned at the appropriate wavelenghts for the red and green dyesAmount of fluorescence emitted (intensity) upon laser excitation ~ amount of mRNA bound to each spotIf the sample in control/experimental condition is in abundance ? green/red, which indicates the relative amount of transcript for the mRNA (EST) in the samples. If both are equal ? yellowIf neither are present ? black;Microarray data generation, processing and analysis;;When miRNA plays an oncogenic role, it targets TSG, control cell differentiation or apoptosis genes, and leads to tumor formation. if miRNA plays the tumor suppressor role, it targets OCG, control cell differentiation or apoptosis genes, so it can suppress tumor formation. Expect negative correlation of miRNA and mRNA expression profiles integrate the human miRNA-targeted (or siRNA-targetd) mRNA data, protein-protein interactions (PPI) records, tissues, pathways, and disease information to establish a disease-related miRNA (or siRNA) pathway database;Introduction – cancer-related miRNAs;A platform for studying miRNAs and cancerous target genes;miRNA, target gene, protein-protein interaction (PPI);Input data and Methods;ResearchProtocol;Term;Results – DEGs predicted by Bioconductor;Results – miRNAs, DEGs and cancer types;Results - The relationship among miR-20a, TGFBR2 and human prostate cancer;A platform for studying miRNAs and cancerous target genes;A platform for studying miRNAs and cancerous target genes;A platform for studying miRNAs and cancerous target genes;Test of hypothesis of PCC and SRC;Results – hsa-m