北大生化课件31.pptVIP

Selenocysteine - the 21st amino acid Prokaryotic selenocysteine incorporation    Working with proteins Methods for separating proteins take advantage of properties such as charge, size, and solubility, which vary from one protein to the next. Because many proteins bind to other biomolecules, proteins can also be separated on the basis of their binding properties. Column chromatography  Ion-exchange chromatography Size-exclusion chromatography Affinity chromatography . . . . . .  谢谢大家! 蛋白质可以通过各种生物化学技术纯化 利用蛋白质的溶解度、净电荷、大小以及与配体结合特异性上的微小差异。