原生质体变育种文.docVIP

PAGE PAGE 7 普那霉素产生菌的原生质体诱变育种 摘要： 普那霉素产生菌始旋链霉菌(Streptomyces pristinaespiralis)11-2在含0．5％甘氨酸的种子培养基中培养到对数生长期，收集菌丝体,经2mg／ml溶菌酶在30℃下作用90min可获得大量的原生质体，其再生率为5．1％。始旋链霉菌11-2原生质体经UV诱变并在含普那霉素的再生平板上筛选普那霉素抗性菌株，从中获得一高产突变株始旋链霉菌ZP-07，普那霉素产量达到1.59g／L，比出发菌株提高101．3％。 关键词： 普那霉素； 原生质体； 诱变； 筛选 Selection of high pristinamycin-producing strain through mutation of protoplast induced by UV radiation ABSTRACT To select a high pristinamycin·-producing strain through protoplast mutation induced by UV radiation，factors affecting protoplast formation and regeneration were studied．Streptornyces pristinaespiralis 11-2 wa