中红外光谱法测定烟草中的木质素-烟草科技.pdf

烟草科技/ Tobacco Science Technology 烟草化学/ Tobacco Chemistry 2014 年第 10 期（总第327 期） 中红外光谱法测定烟草中的木质素 1 1,2 1 *3 3 3 1,2 吴 舜 ，楼宏铭 ，莫贤科 ，孔浩辉 ，黄翼飞 ，王 华 ，邱学青 1. 华南理工大学化学与化工学院，广州市天河区五山路381 号 510641 2. 华南理工大学制浆造纸工程国家重点实验室，广州市天河区五山路381 号 510641 3. 广东中烟工业有限责任公司，广州市荔湾区东沙环翠南路88 号 510385 摘要：采用中红外光谱技术建立了一种原位快速测定烟草木质素含量的方法，并分别测定了烟梗粉和烟 叶粉的木质素含量。利用烟草木质素在1508 cm- 1 的吸收峰建立了烟草木质素的标准曲线。结果表明：标 准曲线的拟合决定系数为0.9914 ；利用该方法测定的“双喜”特色优质白肋烟B 1F 烟梗粉和烟叶粉的木质 素含量分别为2.45% 和1.21% ，相对标准偏差分别为3.88% 和4.17% ，平均加标回收率在96.96%~ 106.01%之 间；该方法操作简便，重现性好，能快速测定烟草中的木质素含量。 关键词：烟草；木质素；中红外光谱；标准曲线法；定量分析 中图分类号：TS411.1 文献标识码：B 文章编号：1002-0861 （2014 ）10-0067-04 Determination of Lignin in Tobacco by Mid-infrared Spectroscopy 1 1,2 1 *3 3 3 1,2 WU Shun , LOU Hongming , MO Xianke , KONG Haohui , HUANG Yifei , WANG Hua , and QIU Xueqing 1. College of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, China 2. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510641, China 3. China Tobacco Guangdong Industrial Co., Ltd., Guangzhou 510385, China Abstract: An in situ method for rapidly determining the lignin content in tobacco was developed with mid-infrared （MIR ）spectrometry, and the lignin contents in ground tobacco stem and leaf were separately determined by this method. The calibration curve of the lignin content in tobacco was obtained on the basis of absorption peak area of lignin at 1508 cm-1 with determination coefficient of 0.9914. The results showed that the lignin contents in the ground stem and leaf of B 1F burley tobacco in “Shuangxi”brand were 2.45% and 1.21% with the relative standard deviations of 3.88% and 4.17% , respectively. The average