第十四章 生物信息学在分子诊断中的作用.ppt

Key Laboratory of Laboratory Medicine, Ministry of Education, China * 五、引物设计 Primer Premier软件: Primer3软件: /cgi-bin/primer/primer3 Oligo、Vector NT、Omiga等 Key Laboratory of Laboratory Medicine, Ministry of Education, China * 六、向数据库提交核酸序列 向EMBL提交数据的网络表格可参见： http://www.ebi.ac.uk/subs/emblsubs.tml 向GenBank数据库提交核酸序列可联网进行 /GenBank/index.html 也可用Sequin软件制作好序列提交文件，向NCBI 发送E-mail(gb-sub@)提交 Key Laboratory of Laboratory Medicine, Ministry of Education, China * Key Laboratory of Laboratory Medicine, Ministry of Education, China * 第五节 蛋白质序列分析 蛋白质基本性质分析 蛋白质结构预测 蛋白质功能预测 Key Laboratory of Laboratory Medicine, Ministry of Education, China * 一、蛋白质基本性质分析 蛋白质的氨基酸组成、分子量、等电点等方面的分析 : OMIGA、DNAMAN、BioEdit、MacVector等 蛋白质疏水性分析 ：ProtScale， /cgi-bin/protscale.pl 预测跨膜区 ： http://genome.cbs.dtu.dk/services/TMHMM-2.0/ /software/TMPRED_form.html http://www.emblheidelberg.de/services/sander/predictprotein ftp://ftp.biochem.ucl.ac.uk。 Key Laboratory of Laboratory Medicine, Ministry of Education, China * Key Laboratory of Laboratory Medicine, Ministry of Education, China * Key Laboratory of Laboratory Medicine, Ministry of Education, China * Key Laboratory of Laboratory Medicine, Ministry of Education, China * Key Laboratory of Laboratory Medicine, Ministry of Education, China * 用TMHMM 软件预测的SARS-CoV 的E蛋白的跨膜区 Key Laboratory of Laboratory Medicine, Ministry of Education, China * 预测信号肽： http://genome.cbs.dtu.dk/services/SignalP/ 蛋白质亚细胞定位 ： http://predict.sanger.ac.uk/nnpsl/ Key Laboratory of Laboratory Medicine, Ministry of Education, China * 预测信号肽 Key Laboratory of Laboratory Medicine, Ministry of Education, China * 预测信号肽 Key Laboratory of Laboratory Medicine, Ministry of Education, China * 蛋白质亚细胞定位 Key Laboratory of Laboratory Medicine, Ministry of Education, China * 蛋白质亚细胞定位 Key Laboratory of Laboratory Medicine, Ministry of Education, China * 二、蛋白质功能预测 蛋白质序列分析和功能预测的一般流程 Key Laboratory of Laboratory Medicine, Ministry of Education, China * 第六节 蛋白质序列分析 二、蛋白质功能预测 磷酸