NMR-核磁共振波谱法.pdf

核磁共振波谱法 Nuclear Magnetic Resonance Spectroscopy，NMR Some history of NMR 1946 Principle of solid state NMR (Bloch, Purcell) 1950 Resonance frequency depends on chemical environment (Proctor, Yu) 1953 Overhauser effect 1956 First NMR spectra of protein (Ribonuclease) 1965 Fourier Transform spectroscopy (Ernst) 1973 Imaging tomography (Mansfield) 1985 First protein structure (bovine pancreatic trypsin inhibitor) in solution (Wüthrich) By now: More than 150 protein structures (M 60 000)