淫羊藿属植物PCR-RFLP遗传多样性研究.pdf

• 562 • 中草药 Chinese Traditional and Herbal Drugs 第 43 卷 第 3 期 2012 年 3 月 淫羊藿属植物 PCR-RFLP 遗传多样性研究 1, 2 3 1* 杜明凤 ，李明军 ，陈庆富 1. 贵州师范大学生命科学学院 植物遗传育种研究所，贵州 贵阳 550001 2. 贵州师范大学 研究生处，贵州 贵阳 550001 3. 贵州省林业调查规划院，贵州 贵阳 550003 摘 要：目的 建立淫羊藿植物 PCR-RFLP 标记系统关系图。方法 对主要来自于四川、贵州的 17 种淫羊藿植物进行了系 统的 PCR-RFLP 标记。结果 在 8 个引物的 PCR-RFLP 标记分析中，有 7 个标记能得到 1 条清晰的谱带；利用 12 种限制性 内切酶对7 个标记的扩增产物消化后，在 84 种引物/酶组合中，共检测到 129 条酶切片段，其中 44 条具有多态性；遗传相 似系数为 0.550～0.988，平均为 0.821 。结论 该属植物遗传关系与其地理分布关系密切。 关键词：淫羊藿属；PCR-RFLP ；遗传多样性; 引物；谱带 中图分类号：R282.12 文献标志码：A 文章编号：0253 - 2670(2012)03 - 0562 - 06 PCR-RFLP genetic diversity of plants in Epimedium L. 1, 2 3 1 DU Ming-feng , LI Ming-jun , CHEN Qing-fu 1. Institute of Plant Genetics and Breeding, School of Life Sciences, Guizhou Normal University, Guiyang 550001, China 2. Department of Postgraduate, Guizhou Normal University, Guiyang 550001, China 3. Guizhou Province Forestry Survey and Planning Institute, Guiyang 550003, China Abstract: Objective To establish the phylogenic map of plants in Epimedium L. based on PCR-RFLP data. Methods Seventeen species from Epimedium L. native to Sichuan and Guizhou provinces etc. were studied by means of PCR-RFLP Marker. Results Among the eight PCR-RFLP Markers, seven (87.5%) could produce one distinct band with no polymorphism detected by direct electrophoresis in 2% agrose gels. After the amplified products were digested by 12 restriction enzymes, a total of 129 bands were detected in 84 PCR-RFLP primer/enzyme combinations. And 44 polymorphic bands accounting for 34.1% have polymorphism. The genetic similarity coefficient (Gs) among 17 species from Epimedium L. showed the range from 0.550 to 0.988, with the mean of 0.821. Conclusion It is clear that there are some relationships between the genetic property and its ge